@article{fdi:010087559,
  title = {{E}xpanding knowledge of methylotrophic capacity : structure and properties of the rough-type lipopolysaccharide from {M}ethylobacterium extorquens and its role on membrane resistance to methanol},
  author = {{D}i {L}orenzo, {F}. and {N}icolardi, {S}. and {M}archetti, {R}. and {V}anacore, {A}. and {G}allucci, {N}. and {D}uda, {K}. and {F}abregat, {F}. {N}. and {N}guyen, {H}. {A}. and {G}ully, {D}jamel and {S}aenz, {J}. and {G}iraud, {E}ric and {P}aduano, {L}. and {M}olinaro, {A}. and {D}'{E}rrico, {G}. and {S}ilipo, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he ability of {M}ethylobacterium extorquens to grow on methanol as the sole carbon and energy source has been the object of intense research activity. {U}nquestionably, the bacterial cell envelope serves as a defensive barrier against such an environmental stressor, with a decisive role played by the membrane lipidome, which is crucial for stress resistance. {H}owever, the chemistry and the function of the main constituent of the {M}. extorquens outer membrane, the lipopolysaccharide ({LPS}), is still undefined. {H}ere, we show that {M}. extorquens produces a rough-type {LPS} with an uncommon, non-phosphory-lated, and extensively {O}-methylated core oligosaccharide, densely substituted with negatively charged residues in the inner region, including novel monosaccharide derivatives such as {O}-methylated {K}do/{K}o units. {L}ipid {A} is composed of a non-phosphorylated trisaccharide backbone with a distinctive, low acylation pattern; indeed, the sugar skeleton was decorated with three acyl moieties and a secondary very long chain fatty acid, in turn substituted by a 3 -{O}-acetyl-butyrate residue. {S}pectroscopic, conformational, and biophysical analyses on {M}. extorquens {LPS} highlighted how structural and tridimensional features impact the molecular organization of the outer membrane. {F}urthermore, these chemical features also impacted and improved membrane resistance in the presence of methanol, thus regulating membrane ordering and dynamics.},
  keywords = {{M}ethylobacterium extorquens ; lipopolysaccharide ({LPS}) ; lipid bilayers ; {NMR} spectroscopy ; {ESI} {FT}-{ICR} mass spectrometry ; lipid {A} {MALDI} {MS} ; electron paramagnetic resonance ({EPR}) ; small-angle neutron scattering ({SANS})},
  booktitle = {},
  journal = {{JACS} {A}u},
  volume = {3},
  numero = {3},
  pages = {929--942},
  year = {2023},
  DOI = {10.1021/jacsau.3c00025},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087559},
}