@article{fdi:010087483,
  title = {{D}evelopment of environmental loop-mediated isothermal amplification (e{LAMP}) diagnostic tool for {B}ulinus truncatus field detection},
  author = {{B}lin, {M}. and {S}enghor, {B}runo and {B}oissier, {J}. and {M}ulero, {S}. and {R}ey, {O}. and {P}ortela, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {G}lobal changes are reshaping the distribution of vector-borne diseases by spreading vectors to previously non-endemic areas. {S}ince 2013, urogenital schistosomiasis has emerged in {C}orsica and threatens {E}uropean countries. {G}astropod vectors release schistosome larvae that can infect humans who come into contact with freshwater bodies. {M}onitoring schistosomiasis host vectors is a prerequisite to understand and subsequently to control this pathogen transmission. {B}ecause malacological surveys are time consuming and require special expertise, the use of a simple molecular method is desirable.{M}ethods {T}he aim of this study is to develop a ready-to-use protocol using the {LAMP} (loop-mediated isothermal amplification) method to detect environmental {DNA} of {B}ulinus truncatus, vector of {S}chistosoma haematobium. {I}nterestingly, {LAMP} method possesses all the characteristics required for adaptability to field conditions particularly in low-income countries: speed, simplicity, lyophilized reagents, low cost and robustness against {DNA} amplification inhibitors. {W}e have tested this new method on {C}orsican water samples previously analysed by q{PCR} and dd{PCR}.{R}esults {W}e demonstrate that our diagnostic tool {B}. truncatus e{LAMP} ({B}t-e{LAMP}) can detect the e{DNA} of {B}ulinus truncatus as effectively as the two other methods. {B}t-e{LAMP} can even detect 1/4 of positive samples not detectable by q{PCR}. {M}oreover, the complete {B}t-e{LAMP} protocol (sampling, sample pre-process, amplification and revelation) does not require sophisticated equipment and can be done in 1 & frac12;h.{C}onclusions {LAMP} detection of environmental {DNA} provides large-scale sensitive surveillance of urogenital schistosomiasis possible by identifying potentially threatened areas. {M}ore generally, e{LAMP} method has great potential in vector-borne diseases and ecology.},
  keywords = {{E}nvironmental {DNA} ; {LAMP} assay ; {I}ntermediate host control ; {B}ulinus truncatus ; {FRANCE} ; {SENEGAL} ; {CORSE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {16},
  numero = {1},
  pages = {78 [11 ]},
  ISSN = {1756-3305},
  year = {2023},
  DOI = {10.1186/s13071-023-05705-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087483},
}