@article{fdi:010087481,
  title = {{CRISPR}i in {X}anthomonas demonstrates functional convergence of transcription activator-like effectors in two divergent pathogens},
  author = {{Z}arate-{C}haves, {C}. {A}. and {A}udran, {C}. and {C}ulma, {C}. {A}. {M}. and {E}scalon, {A}. and {J}avegny, {S}. and {G}agnevin, {L}. and {T}homas, {E}. and {P}impare, {L}. {L}. and {L}opez, {C}. {E}. and {J}acobs, {J}. {M}. and {N}oel, {L}. {D}. and {K}oebnik, {R}alf and {B}ernal, {A}. {J}. and {S}zurek, {B}oris},
  editor = {},
  language = {{ENG}},
  abstract = {{F}unctional analysis of large gene families in plant pathogens can be cumbersome using classical insertional mutagenesis. {A}dditionally, {C}as9 toxicity has limited the application of {CRISPR}-{C}as9 for directed mutagenesis in bacteria.{H}ere, we successfully applied a {CRISPR} interference strategy to investigate the cryptic role of the transcription activator-like effector (tale) multigene family in several plant-pathogenic {X}anthomonas bacterial species, owing to their contribution to pathogen virulence.{S}ingle guide {RNA}s (sg{RNA}s) designed against {X}anthomonas phaseoli pv manihotis tale conserved gene sequences efficiently silenced expression of all tales, with concomitant decrease in virulence and {TALE}-induced host gene expression. {T}he system is readily translatable to other {X}anthomonas species infecting rice, citrus, {B}rassica, and cassava, silencing up to 16 tales in a given strain using a single sg{RNA}. {C}omplementation with plasmid-borne designer tales lacking the sg{RNA}-targeted sequence restored molecular and virulence phenotypes in all pathosystems.{O}ur results evidenced that {X}. campestris pv campestris {CN}08 tales are relevant for symptom development in cauliflower. {T}hey also show that the {M}e{SWEET}10a sugar transporter is surprisingly targeted by the nonvascular cassava pathogen {X}. cassavae, highlighting a new example of {TALE} functional convergence between phylogenetically distant {X}anthomonas. {O}verall, this novel technology provides a platform for discovery and rapid functional understanding of highly conserved gene families.},
  keywords = {bacterial leaf blight of rice ; cassava bacterial blight ; cassava ; bacterial necrosis ; {SWEET} ; transcription activator-like effector ; {X}anthomonas},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[12 p.]},
  ISSN = {0028-646{X}},
  year = {2023},
  DOI = {10.1111/nph.18808},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087481},
}