%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Aubert, J.
%A Bellegarde, F.
%A Oltehua-Lopez, O.
%A Leblanc, Olivier
%A Arteaga-Vazquez, M. A.
%A Martienssen, R. A.
%A Grimanelli, Daniel
%T AGO104 is a RdDM effector of paramutation at the maize b1 locus
%D 2022
%L fdi:010087458
%G ENG
%J PLoS One
%@ 1932-6203
%M ISI:000943102300038
%N 8
%P e0273695 [15 ]
%R 10.1371/journal.pone.0273695
%U https://www.documentation.ird.fr/hor/fdi:010087458
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-04/010087458.pdf
%V 17
%W Horizon (IRD)
%X Although paramutation has been well-studied at a few hallmark loci involved in anthocyanin biosynthesis in maize, the cellular and molecular mechanisms underlying the phenomenon remain largely unknown. Previously described actors of paramutation encode components of the RNA-directed DNA-methylation (RdDM) pathway that participate in the biogenesis of 24-nucleotide small interfering RNAs (24-nt siRNAs) and long non-coding RNAs. In this study, we uncover an ARGONAUTE (AGO) protein as an effector of the RdDM pathway that is in charge of guiding 24-nt siRNAs to their DNA target to create de novo DNA methylation. We combined immunoprecipitation, small RNA sequencing and reverse genetics to, first, validate AGO104 as a member of the RdDM effector complex and, then, investigate its role in paramutation. We found that AGO104 binds 24-nt siRNAs involved in RdDM, including those required for paramutation at the b1 locus. We also show that the ago104-5 mutation causes a partial reversion of the paramutation phenotype at the b1 locus, revealed by intermediate pigmentation levels in stem tissues. Therefore, our results place AGO104 as a new member of the RdDM effector complex that plays a role in paramutation at the b1 locus in maize.
%$ 076 ; 020