@article{fdi:010087458,
  title = {{AGO}104 is a {R}d{DM} effector of paramutation at the maize b1 locus},
  author = {{A}ubert, {J}. and {B}ellegarde, {F}. and {O}ltehua-{L}opez, {O}. and {L}eblanc, {O}livier and {A}rteaga-{V}azquez, {M}. {A}. and {M}artienssen, {R}. {A}. and {G}rimanelli, {D}aniel},
  editor = {},
  language = {{ENG}},
  abstract = {{A}lthough paramutation has been well-studied at a few hallmark loci involved in anthocyanin biosynthesis in maize, the cellular and molecular mechanisms underlying the phenomenon remain largely unknown. {P}reviously described actors of paramutation encode components of the {RNA}-directed {DNA}-methylation ({R}d{DM}) pathway that participate in the biogenesis of 24-nucleotide small interfering {RNA}s (24-nt si{RNA}s) and long non-coding {RNA}s. {I}n this study, we uncover an {ARGONAUTE} ({AGO}) protein as an effector of the {R}d{DM} pathway that is in charge of guiding 24-nt si{RNA}s to their {DNA} target to create de novo {DNA} methylation. {W}e combined immunoprecipitation, small {RNA} sequencing and reverse genetics to, first, validate {AGO}104 as a member of the {R}d{DM} effector complex and, then, investigate its role in paramutation. {W}e found that {AGO}104 binds 24-nt si{RNA}s involved in {R}d{DM}, including those required for paramutation at the b1 locus. {W}e also show that the ago104-5 mutation causes a partial reversion of the paramutation phenotype at the b1 locus, revealed by intermediate pigmentation levels in stem tissues. {T}herefore, our results place {AGO}104 as a new member of the {R}d{DM} effector complex that plays a role in paramutation at the b1 locus in maize.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {17},
  numero = {8},
  pages = {e0273695 [15 ]},
  ISSN = {1932-6203},
  year = {2022},
  DOI = {10.1371/journal.pone.0273695},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087458},
}