Dechavanne S. auteur aut IRD Srivastava A. auteur aut IRD Gangnard S. auteur aut IRD Nunes-Silva S. auteur aut IRD Dechavanne C. auteur aut IRD Fievet Nadine auteur aut IRD Deloron Philippe auteur aut IRD Chene A. auteur aut IRD Gamain B. auteur aut IRD text journalArticle eng text/pdf born digital access Plasmodium falciparum multidomain protein VAR2CSA stands today as the leading vaccine candidate against pregnancy-associated malaria (PAM). Most of the studies aiming to decrypt how naturally acquired immunity develops have assessed the immune recognition of individual VAR2CSA Duffy-binding-like (DBL) domains, thus overlooking the presence of conformational epitopes resulting from the overall folding of the full-length protein. In order to characterize the development of humoral immunity toward VAR2CSA, we made use of a large cohort of 293 Senegalese pregnant women to assess the level of recognition by plasma IgG of the full-length VAR2CSA protein of the 3D7 parasite strain (3D7-VAR2CSA), the CSA-binding multidomains 3D7-DBL1X to -DBL3X (3D7-DBL1X-3X), and the CSA nonbinding multidomains 3D7-DBL4 epsilon to -DBL6 epsilon (3D7-DBL4 epsilon-6 epsilon), as well as individual 3D7-DBL domains. Our results revealed a parity-dependent recognition of the full-length 3D7-VAR2CSA and of the CSA-binding region, 3D7-DBL1X-3X. Indeed, multigravid women possess significantly higher levels of antibodies directed against these constructs than primigravidae. Our results suggest an important role of antibodies targeting the CSA-binding region in the development of immunity against PAM, therefore providing new insights on how natural protection might be acquired and further information for the design of VAR2CSA-based vaccines. specialized 050 052 83 6 2466-2474 2015 0019-9567 https://www.documentation.ird.fr/hor/fdi:010087122 10.1128/iai.03116-14 0019-9567 [F B010087122] https://www.documentation.ird.fr/hor/fdi:010087122 https://www.documentation.ird.fr/intranet/publi/2023-02/010087122.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2015-07-29 2023-02-13 fdi:010087122 fre