@article{fdi:010087036,
  title = {{N}ef-mediated enhancement of virion infectivity and stimulation of viral replication are fundamental properties of primate lentiviruses},
  author = {{M}unch, {J}. and {R}ajan, {D}. and {S}chindler, {M}. and {S}pecht, {A}. and {R}ucker, {E}. and {N}ovembre, {F}. {J}. and {N}errienet, {E}. and {M}ueller {T}rutwin, {M}. {C}. and {P}eeters, {M}artine and {H}ahn, {B}. {H}. and {K}irchhoff, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{N}ef is a multifunctional accessory protein of primate lentiviruses. {R}ecently, it has been shown that the ability of {N}ef to downmodulate {CD}4, {CD}28, and class {I} major histocompatibility complex is highly conserved between most or all primate lentiviruses, whereas {N}ef-mediated downregulation of {T}-cell receptor-{CD}3 was lost in the lineage that gave rise to human immunodeficiency virus type 1 ({HIV}-1). {W}hether or not other {N}ef activities are preserved between different groups of primate lentiviruses remained to be determined. {H}ere, we show that nef genes from a large variety of {HIV}s and simian immunodeficiency viruses ({SIV}s) enhance virion infectivity and stimulate viral replication in human cells and/or in ex vivo infected human lymphoid tissue ({HLT}). {N}otably, nef alleles from unpassaged {SIV}cpz and {SIV}smm enhanced viral infectivity, replication, and cytopathicity in cell culture and in ex vivo infected {HLT} as efficiently as those from {HIV}-1 and {HIV}-2, their human counterparts. {F}urthermore, nef genes from several highly divergent {SIV}s that have not been found in humans were also highly active in human cells and/or tissues. {T}hus, most primate lentiviral {N}efs enhance virion infectivity and stimulate viral replication. {M}oreover, our data show that {SIV}cpz and {SIV}smm {N}efs do not require adaptive changes to perform these functions in human cells or tissues and support the idea that nef alleles from other primate lentiviruses would also be capable of promoting efficient virus spread in humans.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {V}irology},
  volume = {81},
  numero = {24},
  pages = {13852--13864},
  ISSN = {0022-538{X}},
  year = {2007},
  DOI = {10.1128/{JVI}.00904-07},
  URL = {https://www.documentation.ird.fr/hor/fdi:010087036},
}