@article{fdi:010086980,
  title = {{R}ole of two {R}po{N} in {B}radyrhizobium sp. strain {DOA}9 in symbiosis and free-living growth},
  author = {{W}ongdee, {J}. and {P}iromyou, {P}. and {S}ongwattana, {P}. and {G}reetatorn, {T}. and {T}eaumroong, {N}. and {B}oonkerd, {N}. and {G}iraud, {E}ric and {N}ouwen, {N}ico and {T}ittabutr, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}po{N} is an alternative sigma factor (sigma 54) that recruits the core {RNA} polymerase to promoters of genes. {I}n bacteria, {R}po{N} has diverse physiological functions. {I}n rhizobia, {R}po{N} plays a key role in the transcription of nitrogen fixation (nif) genes. {T}he {B}radyrhizobium sp. {DOA}9 strain contains a chromosomal (c) and plasmid (p) encoded {R}po{N} protein. {W}e used single and double rpo{N} mutants and reporter strains to investigate the role of the two {R}po{N} proteins under free-living and symbiotic conditions. {W}e observed that the inactivation of rpo{N}c or rpo{N}p severely impacts the physiology of the bacteria under free-living conditions, such as the bacterial motility, carbon and nitrogen utilization profiles, exopolysaccharide ({EPS}) production, and biofilm formation. {H}owever, free-living nitrogen fixation appears to be under the primary control of {R}po{N}c. {I}nterestingly, drastic effects of rpo{N}c and rpo{N}p mutations were also observed during symbiosis with {A}eschynomene americana. {I}ndeed, inoculation with rpo{N}p, rpo{N}c, and double rpo{N} mutant strains resulted in decreases of 39, 64, and 82% in the number of nodules, respectively, as well as a reduction in nitrogen fixation efficiency and a loss of the bacterium's ability to survive intracellularly. {T}aken together, the results show that the chromosomal and plasmid encoded {R}po{N} proteins in the {DOA}9 strain both play a pleiotropic role during free-living and symbiotic states.},
  keywords = {{B}radyrhizobium ; {R}po{N} ; nitrogen fixation ; free-living ; symbiosis ; nodulation ; extracellular polysaccharide},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {14},
  numero = {},
  pages = {1131860 [12 ]},
  year = {2023},
  DOI = {10.3389/fmicb.2023.1131860},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086980},
}