@article{fdi:010086947,
  title = {{D}iterpenes of {C}offea seeds show antifungal and anti-insect activities and are transferred from the endosperm to the seedling after germination},
  author = {{A}ntoine, {G}. and {V}aissayre, {V}irginie and {M}eile, {J}. {C}. and {P}ayet, {J}. and {C}onejero, {G}. and {C}ostet, {L}. and {F}ock-{B}astide, {I}. and {J}o{\¨e}t, {T}hierry and {D}ussert, {S}t{\'e}phane},
  editor = {},
  language = {{ENG}},
  abstract = {{S}pecies of the genus {C}offea accumulate diterpenes of the ent-kaurane family in the endosperm of their seeds, of which cafestol and kahweol are the most abundant. {T}he diterpenes are mainly stored in esterified form with fatty acids, mostly palmitate. {I}n contrast to the numerous studies on their effects on human health and therapeutic applications, nothing was previously known about their biological and ecological role in planta. {T}he antifungal and anti-insect activities of cafestol and cafestol palmitate were thus investigated in this study. {C}afestol signif-icantly affected the mycelial growth of five of the six phytopathogenic fungi tested. {I}t also greatly reduced the percentage of pupation of larvae and the pupae and adult masses of one of the two fruit flies tested. {B}y contrast, cafestol palmitate had no significant effect against any of the fungi and insects studied. {U}sing confocal imaging and oil body isolation and analysis, we showed that diterpenes are localized in endosperm oil bodies, suggesting that esterification with fatty acids enables the accumulation of large amounts of diterpenes in a non-toxic form. {D}iterpene measurements in all organs of seedlings recovered from whole seed germination or embryos isolated from the endosperm showed that diterpenes are transferred from the endosperm to the cotyledons during seedling growth and then distributed to all organs, including the hypocotyl and the root. {C}ollectively, our findings show that coffee diterpenes are broad-spectrum defence compounds that protect not only the seed on the mother plant and in the soil, but also the seedling after germination.},
  keywords = {{C}offea arabica ; {C}offea mauritiana ; {R}ubiaceae ; {B}iological activity ; {HPLC} ; {GC} ; {M}icroscopy ; {C}afestol ; {K}ahweol ; {E}nt-{K}aurane diterpenoids},
  booktitle = {},
  journal = {{P}lant {P}hysiology and {B}iochemistry},
  volume = {194},
  numero = {},
  pages = {627--637},
  ISSN = {0981-9428},
  year = {2023},
  DOI = {10.1016/j.plaphy.2022.12.013},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086947},
}