@article{fdi:010086923,
  title = {{C}arbonyl cyanide 3-chloro phenyl hydrazone ({CCCP}) restores the colistin sensitivity in {B}rucella intermedia},
  author = {{Z}oaiter, {M}. and {Z}eaiter, {Z}. and {M}ediannikov, {O}leg and {S}okhna, {C}heikh and {F}ournier, {P}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}rucella intermedia (formerly {O}chrobactrum intermedium), a non-fermentative bacterium, has been isolated from animals and human clinical specimens. {I}t is naturally resistant to polymyxins, including colistin ({CO}), and may cause opportunistic infections in humans. {W}e isolated six {B}rucella intermedia strains from {S}enegalese monkey stool. {I}n order to determine whether an efflux pump mechanism was involved in {CO} resistance in {B}. intermedia, we evaluated the effects of verapamil ({VRP}), reserpine ({RSP}), phe-arg beta-naphthylamide dihydrochloride ({PA} beta {N}) and carbonyl cyanide 3-chloro phenyl hydrazone ({CCCP}), four efflux pump inhibitors, on these colistin-resistant strains. {U}sing the broth microdilution method, a {CO} and {CCCP} combination of 2 mu g/m{L} and 10 mu g/m{L}, respectively, significantly reduced the {CO} minimal inhibitory concentration ({MIC}) of {B}. intermedia, supporting an efflux pump mechanism. {I}n contrast, {VRP}, {PA} beta {N} and {RSP} did not restore {CO} susceptibility. {A} time kill assay showed a bactericidal effect of the {CO}-{CCCP} combination. {G}enomic analysis revealed a potential implication in the {CO} resistance mechanism of some conserved efflux pumps, such as {Y}ej{ABEF}, {N}or{M} and {E}mr{AB}, as previously reported in other bacteria. {A}n inhibitory effect of the {CO}-{CCCP} combination was observed on biofilm formation using the crystal violet method. {T}hese results suggest that the intrinsic {CO} resistance in {B}rucella intermedia is linked to an efflux pump mechanism and that the synergistic effect of {CO}-{CCCP} may open a new field to identify new treatments to restore antibiotic efficacy in humans.},
  keywords = {intrinsic colistin resistance ; efflux pump inhibitors ; carbonyl cyanide ; 3-chloro phenyl hydrazone ; {CO}-{MIC} ; synergy ; efflux pump ; biofilm formation},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {M}olecular {S}ciences},
  volume = {24},
  numero = {3},
  pages = {2106 [14 ]},
  year = {2023},
  DOI = {10.3390/ijms24032106},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086923},
}