@article{fdi:010086886,
  title = {{A} new {WHO} bottle bioassay method to assess the susceptibility of mosquito vectors to public health insecticides : results from a {WHO}-coordinated multi-centre study},
  author = {{C}orbel, {V}incent and {K}ont, {M}. {D}. and {A}humada, {M}. {L}. and {A}ndreo, {L}aura and {B}ayili, {B}. and {B}ayili, {K}. and {B}rooke, {B}. and {C}aballero, {J}. {A}. {P}. and {L}ambert, {B}. and {C}hurcher, {T}. {S}. and {D}uchon, {S}t{\'e}phane and {E}tang, {J}. and {F}lores, {A}. {E}. and {G}unasekaran, {K}. and {J}untarajumnong, {W}. and {K}irby, {M}. and {D}avies, {R}. and {L}ees, {R}. {S}. and {L}enhart, {A}. and {L}ima, {J}. {B}. {P}. and {M}artins, {A}. {J}. and {M}ueller, {P}. and {N}'{G}uessan, {R}. and {N}gufor, {C}. and {P}raulins, {G}. and {Q}uinones, {M}. and {R}aghavendra, {K}. and {V}erma, {V}. and {R}us, {A}. {C}. and {S}amuel, {M}. and {Y}ing, {K}. {S}. and {S}ungvornyothin, {S}. and {U}ragayala, {S}. and {V}elayudhan, {R}. and {Y}adav, {R}. {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he continued spread of insecticide resistance in mosquito vectors of malaria and arboviral diseases may lead to operational failure of insecticide-based interventions if resistance is not monitored and managed efficiently. {T}his study aimed to develop and validate a new {WHO} glass bottle bioassay method as an alternative to the {WHO} standard insecticide tube test to monitor mosquito susceptibility to new public health insecticides with particular modes of action, physical properties or both.{M}ethods {A} multi-centre study involving 21 laboratories worldwide generated data on the susceptibility of seven mosquito species ({A}edes aegypti, {A}edes albopictus, {A}nopheles gambiae sensu stricto [{A}n. gambiae s.s.], {A}nopheles funestus, {A}nopheles stephensi, {A}nopheles minimus and {A}nopheles albimanus) to seven public health insecticides in five classes, including pyrethroids (metofluthrin, prallethrin and transfluthrin), neonicotinoids (clothianidin), pyrroles (chlorfenapyr), juvenile hormone mimics (pyriproxyfen) and butenolides (flupyradifurone), in glass bottle assays. {T}he data were analysed using a {B}ayesian binomial model to determine the concentration-response curves for each insecticide-species combination and to assess the within-bioassay variability in the susceptibility endpoints, namely the concentration that kills 50% and 99% of the test population ({LC}50 and {LC}99, respectively) and the concentration that inhibits oviposition of the test population by 50% and 99% ({OI}50 and {OI}99), to measure mortality and the sterilizing effect, respectively.{R}esults {O}verall, about 200,000 mosquitoes were tested with the new bottle bioassay, and {LC}50/{LC}99 or {OI}50/{OI}99 values were determined for all insecticides. {V}ariation was seen between laboratories in estimates for some mosquito species-insecticide combinations, while other test results were consistent. {T}he variation was generally greater with transfluthrin and flupyradifurone than with the other compounds tested, especially against {A}nopheles species. {O}verall, the mean within-bioassay variability in mortality and oviposition inhibition were < 10% for most mosquito species-insecticide combinations.{C}onclusion {O}ur findings, based on the largest susceptibility dataset ever produced on mosquitoes, showed that the new {WHO} bottle bioassay is adequate for evaluating mosquito susceptibility to new and promising public health insecticides currently deployed for vector control. {T}he datasets presented in this study have been used recently by the {WHO} to establish 17 new insecticide discriminating concentrations ({DC}s) for either {A}edes spp. or {A}nopheles spp. {T}he bottle bioassay and {DC}s can now be widely used to monitor baseline insecticide susceptibility of wild populations of vectors of malaria and {A}edes-borne diseases worldwide.},
  keywords = {{A}edes ; {A}nopheles ; {I}nsecticide resistance ; {P}ublic health insecticides ; {S}usceptibility test ; {V}ector control ; {WHO} bottle bioassay ; {D}iscriminating concentrations ; {MONDE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {16},
  numero = {1},
  pages = {21 [17 p.]},
  ISSN = {1756-3305},
  year = {2023},
  DOI = {10.1186/s13071-022-05554-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086886},
}