Mohamed H. S. auteur aut IRD Aboubaker M. H. auteur aut IRD Dumont Y. auteur aut IRD Didelot M. N. auteur aut IRD Michon A. L. auteur aut IRD Galal Lokman auteur aut IRD Jean-Pierre H. auteur aut IRD Godreuil S. auteur aut IRD text journalArticle eng text/pdf reformatted digital access The emergence and spread of multidrug resistant Enterobacterales (MDR-E) are a global public health issue. This problem also concerns urinary tract infections (UTI), which are the second most frequent infections after respiratory infections. The objective of this study was to determine MDR-E frequency and to characterize MDR-E isolates from patients with community-acquired UTIs in Djibouti, Republic of Djibouti. From 800 clinical urinary samples collected at the Mer Rouge Laboratory, Djibouti, from January to July 2019, 142 were identified as Enterobacterales (age range of the 142 patients mean age is 42 years.) Mass spectrometry analysis of these isolates identified 117 Escherichia coli, 14 Klebsiella pneumoniae, 2 Proteus mirabilis, 4 Enterobacter spp., 4 Providencia stuartii and 1 Franconibacter helveticus. Antibiotic susceptibility testing (disk diffusion method) of these 142 isolates detected 68 MDR-E (68/142 = 48%): 65 extended-spectrum beta lactamase- (ESBL), 2 carbapenemase- (one also ESBL), and 1 cephalosporinase-producer. Multiplex PCR and sequencing showed that the 65 ESBL-producing isolates carried genes encoding CTX-M enzymes (CTX-M-15 in 97% and CTX-M-9 in 3% of isolates). Two isolates harboured a gene encoding the OXA-48-like carbapenemase, and one the gene encoding the AmpC CMY-2 cephalosporinase. Genes implicated in resistance to quinolones (qnrB, aac (6 ')-Ib-cr, qnrD, oqxA and B) also were detected. Among the E. coli phylogroups, B2 was the most common phylogenetic group (21% of MDR-E isolates and 26% of non-MDR-E isolates), followed by A (14% and 12%), B1 (9% and 7%), D (3% and 3%), F (3% and 3%) and E (2% and 2%). This study highlights the high frequency of ESBL producers and the emergence of carbapenemase-producers among Enterobacterales causing community-acquired UTIs in Djibouti. specialized urinary tract infections Enterobacterales extended-spectrum beta-lactamase carbapenemases community Djibouti DJIBOUTI 050 084 11 12 1740 [10 ] 2022 2079-6382 https://www.documentation.ird.fr/hor/fdi:010086742 10.3390/antibiotics11121740 2079-6382 [F B010086742] https://www.documentation.ird.fr/hor/fdi:010086742 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2023-02/010086742.pdf IRD - Base Horizon / Pleins textes 2023-02-08 2025-02-24 fdi:010086742 fre