@article{fdi:010086729,
  title = {{C}ell type variability in the incorporation of lipids in the dengue virus virion},
  author = {{H}itakarun, {A}. and {W}illiamson, {M}. {K}. and {Y}impring, {N}. and {S}ornjai, {W}. and {W}ikan, {N}. and {A}rthur, {C}. {J}. and {P}ompon, {J}ulien and {D}avidson, {A}. {D}. and {S}mith, {D}. {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus ({DENV}) virion and is crucial for virus entry. {D}espite its significance, the virion's lipid composition is still poorly understood. {I}n tandem with lipid profiles of the cells utilised to generate the virions, this work determined a partial lipid profile of {DENV} virions derived from two cell lines ({C}6/36 and {LLC}-{MK}2). {T}he results showed distinctive profiles between the two cell types. {I}n the mammalian {LLC}-{MK}2 cells, 30.8% (73/237 identified lipid species; 31 upregulated, 42 downregulated) of lipid species were altered in response to infection, whilst in insect {C}6/36 cells only 12.0% (25/208; 19 upregulated, 6 downregulated) of lipid species showed alterations in response to infection. {F}or virions from {LLC}-{MK}2 cells, 14 lipids were detected specifically in virions with a further seven lipids being enriched (over mock controls). {F}or virions from {C}6/36 cells, 43 lipids were detected that were not seen in mock preparations, with a further 16 being specifically enriched (over mock control). {T}hese results provide the first lipid description of {DENV} virions produced in mammalian and mosquito cells, as well as the lipid changes in the corresponding infected cells.},
  keywords = {dengue virus ; glycerolipids ; sphingolipids ; glycerophospholipids ; {LLC}-{MK}2 cells ; {C}6 ; 36 cells ; virion ; lipid},
  booktitle = {},
  journal = {{V}iruses},
  volume = {14},
  numero = {11},
  pages = {2566 [17 p.]},
  year = {2022},
  DOI = {10.3390/v14112566},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086729},
}