Kubina J. auteur aut IRD Hily J. M. auteur aut IRD Mustin P. auteur aut IRD Komar V. auteur aut IRD Garcia S. auteur aut IRD Martin I. R. auteur aut IRD Poulicard Nils auteur aut IRD Velt A. auteur aut IRD Bonnet V. auteur aut IRD Mercier L. auteur aut IRD Lemaire O. auteur aut IRD Vigne E. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Fanleaf degeneration is a complex viral disease of Vitis spp. that detrimentally impacts fruit yield and reduces the productive lifespan of most vineyards worldwide. In France, its main causal agent is grapevine fanleaf virus (GFLV). In the past, field experiments were conducted to explore cross-protection as a management strategy of fanleaf degeneration, but results were unsatisfactory because the mild virus strain negatively impacted fruit yield. In order to select new mild GFLV isolates, we examined two old 'Chardonnay' parcels harbouring vines with distinct phenotypes. Symptoms and agronomic performances were monitored over the four-year study on 21 individual vines that were classified into three categories: asymptomatic GFLV-free vines, GFLV-infected vines severely diseased and GFLV-infected vines displaying mild symptoms. The complete coding genomic sequences of GFLV isolates in infected vines was determined by high-throughput sequencing. Most grapevines were infected with multiple genetically divergent variants. While no specific molecular features were apparent for GFLV isolates from vines displaying mild symptoms, a genetic differentiation of GFLV populations depending on the vineyard parcel was observed. The mild symptomatic grapevines identified during this study were established in a greenhouse to recover GFLV variants of potential interest for cross-protection studies. specialized grapevine fanleaf virus fanleaf degeneration grapevine symptomatology mild isolates cross-protection genetic and phenotypic diversity high throughput sequencing virome FRANCE 076 14 10 2303 [21 ] 2022 https://www.documentation.ird.fr/hor/fdi:010086425 10.3390/v14102303 [F B010086425] https://www.documentation.ird.fr/hor/fdi:010086425 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-12/010086425.pdf IRD - Base Horizon / Pleins textes 2022-12-09 2025-02-24 fdi:010086425 fre