@article{fdi:010086425,
  title = {{C}haracterization of grapevine fanleaf virus isolates in '{C}hardonnay' vines exhibiting severe and mild symptoms in two vineyards},
  author = {{K}ubina, {J}. and {H}ily, {J}. {M}. and {M}ustin, {P}. and {K}omar, {V}. and {G}arcia, {S}. and {M}artin, {I}. {R}. and {P}oulicard, {N}ils and {V}elt, {A}. and {B}onnet, {V}. and {M}ercier, {L}. and {L}emaire, {O}. and {V}igne, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}anleaf degeneration is a complex viral disease of {V}itis spp. that detrimentally impacts fruit yield and reduces the productive lifespan of most vineyards worldwide. {I}n {F}rance, its main causal agent is grapevine fanleaf virus ({GFLV}). {I}n the past, field experiments were conducted to explore cross-protection as a management strategy of fanleaf degeneration, but results were unsatisfactory because the mild virus strain negatively impacted fruit yield. {I}n order to select new mild {GFLV} isolates, we examined two old '{C}hardonnay' parcels harbouring vines with distinct phenotypes. {S}ymptoms and agronomic performances were monitored over the four-year study on 21 individual vines that were classified into three categories: asymptomatic {GFLV}-free vines, {GFLV}-infected vines severely diseased and {GFLV}-infected vines displaying mild symptoms. {T}he complete coding genomic sequences of {GFLV} isolates in infected vines was determined by high-throughput sequencing. {M}ost grapevines were infected with multiple genetically divergent variants. {W}hile no specific molecular features were apparent for {GFLV} isolates from vines displaying mild symptoms, a genetic differentiation of {GFLV} populations depending on the vineyard parcel was observed. {T}he mild symptomatic grapevines identified during this study were established in a greenhouse to recover {GFLV} variants of potential interest for cross-protection studies.},
  keywords = {grapevine fanleaf virus ; fanleaf degeneration ; grapevine ; symptomatology ; mild isolates ; cross-protection ; genetic and phenotypic ; diversity ; high throughput sequencing ; virome ; {FRANCE}},
  booktitle = {},
  journal = {{V}iruses},
  volume = {14},
  numero = {10},
  pages = {2303 [21 p.]},
  year = {2022},
  DOI = {10.3390/v14102303},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086425},
}