@article{fdi:010086421,
  title = {{D}etection of tick-borne pathogens in ticks from cattle in western highlands of {C}ameroon},
  author = {{N}gnindji-{Y}oudje, {Y}. and {D}iarra, {A}dama {Z}an and {L}ontsi-{D}emano, {M}. and {T}chuinkam, {T}. and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study aimed to detect and identify microorganisms in ticks collected in the {W}estern {H}ighlands of {C}ameroon. {Q}uantitative real-time and standard {PCR} assays, coupled with sequencing, were used. {A} total of 944 ticks collected from cattle in five distinct sites in {C}ameroon were selected for the analyses. {T}hey belonged to five genera ({A}mblyomma, {H}yalomma, {R}hipicephalus, {H}aemaphysalis, and {I}xodes) and twelve species. {R}eal-time {PCR} revealed that 23% (n = 218) of the ticks were positive for {R}ickettsia spp., 15% (n = 141) for bacteria of the {A}naplasmataceae family, 3% (n = 29) for {P}iroplasmida, 0.5% (n = 5) for {C}oxiella burnetii, 0.4% (n = 4) for {B}orrelia spp., and 0.2% (n = 2) for {B}artonella spp. {T}he co-infection rate (3.4%, n = 32) involved mainly {R}ickettsia spp. and {A}naplasmataceae. {O}f the {R}ickettsia spp. positive ticks, the targeted {PCR} and sequencing yielded {R}ickettsia africae (78.9%), {R}ickettsia aeschlimannii (6.4%), {R}ickettsia massiliae (7.8%), {C}andidatus {R}ickettsia barbariae (0.9%), and {R}ickettsia sp. (0.9%). {A}naplasmataceae included {A}naplasma marginale (4.3%), {A}naplasma platys (1.4%), {A}naplasma centrale (0.7%), {E}hrlichia ruminantium (0.7%), {W}olbachia sp., {C}andidatus {E}hrlichia rustica (13.5%), {C}andidatus {E}hrlichia urmitei (7%), and an uncultured {E}hrlichia sp. (4.2%). {B}orrelia theileri was identified in one {R}hipicephalus microplus tick. {U}nfortunately, {P}iroplasmida could not be identified to the species level. {T}his study demonstrates that in {C}ameroon, ticks harbour a wide variety of microorganisms and present a risk of zoonotic diseases.},
  keywords = {tick-borne pathogen ; zoonoses ; cattle ; ticks ; {PCR} ; {C}ameroon ; {CAMEROUN}},
  booktitle = {},
  journal = {{M}icroorganisms},
  volume = {10},
  numero = {10},
  pages = {1957 [15 ]},
  year = {2022},
  DOI = {10.3390/microorganisms10101957},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086421},
}