@article{fdi:010086136,
  title = {{A}dapting field-mosquito collection techniques in a perspective of near-infrared spectroscopy implementation},
  author = {{S}ome, {B}. {M}. and {D}a, {D}. {F}. and {M}c{C}abe, {R}. and {D}jegbe, {N}. {D}. {C}. and {P}are, {L}. {I}. {G}. and {W}erme, {K}. and {M}ouline, {K}arine and {L}ef{\`e}vre, {T}. and {O}uedraogo, {A}. {G}. and {C}hurcher, {T}. {S}. and {D}abire, {R}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {N}ear-infrared spectroscopy ({NIRS}) has the potential to be a useful tool for assessing key entomological parameters of malaria-transmitting mosquitoes, including age, infectious status and species identity. {H}owever, before {NIRS} can be reliably used in the field at scale, methods for killing mosquitoes and conserving samples prior to {NIRS} scanning need to be further optimized. {H}istorically, mosquitoes used in studies have been killed with chloroform, although this approach is not without health hazards and should not be used in human dwellings. {F}or the application of {NIRS} scanning it is also unclear which mosquito preservation method to use. {T}he aim of the study reported here was to investigate the use of pyrethrum spray, a commercially available insecticide spray in {B}urkina {F}aso, for killing mosquitoes {M}ethods: {L}aboratory-reared {A}nopheles gambiae and {A}nopheles coluzzii were killed using either a pyrethrum insecticide spray routinely used in studies involving indoor mosquito collections ({K}altox {P}aalga ({R}); {S}aphyto, {B}obo-{D}ioulasso, {B}urkina {F}aso) or chloroform ("gold standard"). {P}reservative methods were also investigated to determine their impact on {NIRS} accuracy in predicting the species of laboratory-reared {A}nopheles and wild-caught mosquito species. {A}fter analysis of fresh samples, mosquitoes were stored in 80% ethanol or in silica gel for 2 weeks and re-analyzed by {NIRS}. {I}n addition, experimentally infected {A}n. coluzzii and wild-caught {A}n. gambiae sensu lato (s.l.) were scanned as fresh samples to determine whether they contained sporozoites, then stored in the preservatives mentioned above for 2 weeks before being re-analyzed. {R}esults: {T}he difference in the accuracy of {NIRS} to differentiate between laboratory-reared {A}n. gambiae mosquitoes and {A}n. coluzzii mosquitoes killed with either insecticide (90%) or chloroform (92%) was not substantial. {NIRS} had an accuracy of 90% in determining mosquito species for mosquitoes killed with chloroform and preserved in ethanol or silica gel. {T}he accuracy was the same when the pyrethrum spray was used to kill mosquitoes followed by preservation in silica gel, but was lower when ethanol was used as a preservative (80%). {R}egarding infection status, {NIRS} was able to differentiate between infected and uninfected mosquitoes, with a slightly lower accuracy for both laboratory and wild-caught mosquitoes preserved in silica gel or ethanol. {C}onclusions: {T}he results show that {NIRS} can be used to classify {A}n. gambiae s.l. species killed by pyrethrum spray with no loss of accuracy. {T}his insecticide may have practical advantages over chloroform for the killing of mosquitoes in {NIRS} analysis.},
  keywords = {{N}ear-infrared spectroscopy ; {A}nopheles ; {P}yrethrum spray ; {P}lasmodium falciparum ; {C}hloroform},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {15},
  numero = {1},
  pages = {338 [10 ]},
  ISSN = {1756-3305},
  year = {2022},
  DOI = {10.1186/s13071-022-05458-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086136},
}