@article{fdi:010086034,
  title = {{R}hodnius ({S}tal, 1859) ({H}emiptera, {T}riatominae) genus in {B}olivian {A}mazonia : a risk for human populations ?},
  author = {{D}epickere, {S}. and {V}illacis, {A}. {G}. and {S}antillan-{G}uayasamin, {S}. and {R}afael, {J}. {E}. {C}. and {B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique and {Z}epita, {S}. {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}hagas disease, one of the most important neglected tropical diseases in the countries of {L}atin {A}merica, is considered to be a particularly important public health concern in the {A}mazon region due to increases in the number of outbreaks of acute {C}hagas disease and increased local transmission in the last 20 years. {H}owever, relative to other countries, in {B}olivia there is little information available on its transmission in the {A}mazon region. {T}he aim of this study was to investigate the infestation of palm trees, the main habitat of {T}riatominae in the region, in several localities, to evaluate the danger they represent to inhabitants. {M}ethods: {T}riatominae were collected using live bait traps left overnight in six localities in {P}ando and {B}eni {D}epartments, {B}olivia. {DNA} extraction and sequencing were used to establish the {T}riatominae species ({C}ytb, 16{S} and 28{S}-{D}2 gene fragments), and the blood meal sources ({C}ytb fragment). {T}rypanosoma sp. infection was analyzed by sequencing gene fragments ({GPX}, {GPI}, {HMCOAR}, {LAP}, {PDH} and {COII}) or by mini-exon multiplex {PCR}. {R}esults: {A} total of 325 {R}hodnius were captured (97.3% of nymphs) from the 1200 traps placed in 238 palm trees and 32 burrows/ground holes. {S}equence analyses on {DNA} extracted from 114 insects and phylogeny analysis identified two triatomine species: {R}hodnius stali (17%) and {R}hodnius montenegrensis (equated to {R}hodnius robustus {II}, 83%). {T}hese were found in palm trees of the genera {A}ttalea (69%), {A}strocaryum (13%), {C}opernicia (12%), {E}uterpe (2%) and {A}crocomia (1%). {T}he infection rate was around 30% (165 analyzed insects), with 90% of analyzed insects infected by {T}rypanosoma cruzi (only the {T}c{I} discrete typing unit was detected), 3% infected by {T}rypanosoma rangeli (first time found in {B}olivian {T}riatominae) and 7% infected by mixed {T}. cruzi ({T}c{I})-{T}. rangeli. {R}hodnius specimens fed on {D}idelphidae, rodents, gecko and humans. {C}onclusions: {T}he results of this study highlight the epidemiological importance of {R}hodnius in the {B}olivian {A}mazon region. {T}he huge geographical distribution of {R}hodnius and their proximity to the human dwellings, high infection rate and frequent meals on the human population highlight a risk of transmission of {C}hagas disease in the region.},
  keywords = {{C}hagas ; {T}riatominae ; {R}hodnius stali ; {R}hodnius montenegrensis ; {T}rypanosoma cruzi ; {DTU}s ; {B}lood meal origin ; {B}olivia ; {A}mazonia ; {G}eographic distribution ; {BOLIVIE} ; {AMAZONIE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {15},
  numero = {1},
  pages = {307 [22 p.]},
  ISSN = {1756-3305},
  year = {2022},
  DOI = {10.1186/s13071-022-05423-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086034},
}