@article{fdi:010086027, title = {{F}ish diversity along the {M}ekong {R}iver and {D}elta inferred by environmental-{DNA} in a period of dam building and downstream salinization}, author = {{D}urand, {J}ean-{D}ominique and {S}imier, {M}onique and {T}ran, {N}. {T}. and {G}rudpan, {C}. and {C}han, {B}. {Y}. and {N}guyen, {B}. {N}. {L}. and {H}oang, {H}. {D}. and {P}anfili, {J}acques}, editor = {}, language = {{ENG}}, abstract = {{T}he {M}ekong {R}iver is one of the largest rivers in the world and hosts the second greatest fish diversity in the world after the {A}mazon. {H}owever, despite the importance of this diversity and its associated biomass for human food security and the economy, different anthropogenic pressures threaten the sustainability of the {M}ekong {R}iver and fish diversity, including the intense damming of the main river. {B}oth the increase in salt-water penetration into the {M}ekong {D}elta and the disrupted connectivity of the river may have serious impacts on the numerous freshwater and migratory species. {T}o evaluate the potential of an e{DNA} approach for monitoring fish diversity, water was sampled at 15 sites along the salinity gradient in the {M}ekong {D}elta and along 1500 km of the main stream, from {V}ietnam to {T}hailand and {L}aos. {A} total of 287 {OTU}s were recovered, of which 158 were identified to the species level using both reference sequences available in {G}en{B}ank and references obtained locally. {A}gglomerative hierarchical clustering and {PCA} identified up to three main species assemblages in our samples. {I}f the transition from brackish to freshwater conditions represents the main barrier between two of these assemblages, more surprisingly, the two other assemblages were observed in the freshwater {M}ekong, with a spatial disjunction that did not match any biogeographic ecoregion or the {K}hone falls, the latter thought to be an important fish dispersion barrier. {B}etween 60% and 95% of the freshwater species were potamodromous. {T}his pioneer e{DNA} study in the {M}ekong {R}iver at this geographical and ecological scale clearly confirmed the potential of this approach for ecological and diversity monitoring. {I}t also demonstrated the need to rapidly build an exhaustive {M}ekong fish barcode library to enable more accurate species' assignment. {M}ore e{DNA} surveys can now be expected to better describe the ecological niche of different species, which is crucial for any models aimed at predicting the impact of future damming of the {M}ekong.}, keywords = {aquatic diversity monitoring ; anthropogenetic pressures ; fish species ; assemblage ; niche ; spatial distribution ; {LAOS} ; {THAILANDE} ; {CAMBODGE} ; {VIET} {NAM} ; {MEKONG} {COURS} {D}'{EAU} ; {MEKONG} {DELTA}}, booktitle = {}, journal = {{D}iversity}, volume = {14}, numero = {8}, pages = {634 [21 ]}, year = {2022}, DOI = {10.3390/d14080634}, URL = {https://www.documentation.ird.fr/hor/fdi:010086027}, }