@article{fdi:010086016,
  title = {{M}olecular typing reveals distinct {M}ycoplasma genitalium transmission networks among a cohort of men who have sex with men and a cohort of women in {F}rance},
  author = {{G}uiraud, {J}. and {H}elary, {M}. and {L}e {R}oy, {C}. and {E}lguero, {E}ric and {P}ereyre, {S}. and {B}ebear, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ycoplasma genitalium causes sexually transmitted infecti.ons in men and women. {T}reatment failures to macrolides and fluoroquinolones have been reported worldwide. {A}lthough the mgp{B} typing method has often been used in {M}. genitalium-infected men who have sex with men ({MSM}), limited typing data are available for {M}. genitalium-infected women. {I}n this study, we aimed to investigate the genetic relationship between {M}. genitalium strains and their antibiotic resistance profile in a cohort of {MSM} (86.2% on {HIV} preexposure prophylaxis [{P}r{EP}], 13.8% {HIV} positive) and a large cohort of women using mgp{B}/{MG}309 typing. {T}he mgp{B} types were determined in 374 samples from 305 women and 65 {MSM}. {T}hree {MSM} and one woman had two concurrent or subsequent samples. {M}acrolide and fluoroquinolone resistance-associated mutations were searched in the 23{S} r{RNA} as well as par{C} and gyr{A} genes. {T}he mgp{B} phylogenetic construction revealed three large clusters that differed according to sexual practices and geographical origin of patients. {T}he prevalence of macrolide and fluoroquinolone resistance was significantly higher in {MSM} compared with women (95.4% vs. 14.1% and 30.6% vs. 7.2%, p < 0.001, respectively). {T}he macrolide resistance spread was polyclonal in both populations, but clonal diffusion of two dual-resistant types was observed in {P}r{EP} users in association with high antibiotic pressure and dense connectivity in this population.},
  keywords = {{M}ycoplasma genitalium ; antimicrobial resistance ; sexually transmitted infection ; macrolides ; fluoroquinolones ; mgp{B} ; {MG}309 ; typing ; {FRANCE}},
  booktitle = {},
  journal = {{M}icroorganisms},
  volume = {10},
  numero = {8},
  pages = {1587 [11 p.]},
  year = {2022},
  DOI = {10.3390/microorganisms10081587},
  URL = {https://www.documentation.ird.fr/hor/fdi:010086016},
}