@article{fdi:010085913,
  title = {{U}nveiling biogeographical patterns of the ichthyofauna in the {T}uichi basin, a biodiversity hotspot in the {B}olivian {A}mazon, using environmental {DNA}},
  author = {{M}ariac, {C}{\'e}dric and {D}uponchelle, {F}abrice and {M}iranda, {G}. and {R}amallo, {C}. and {W}allace, {R}. and {T}arifa, {G}. and {G}arcia-{D}avila, {C}. and {O}rtega, {H}. and {P}into, {J}. and {R}enno, {J}ean-{F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{T}o date, more than 2400 valid fish species have been recorded in the {A}mazon basin. {H}owever, some regions remain poorly documented. {T}his is the case in the {B}eni basin and in particular in one of its main sub-basins, the {T}uichi, an {A}ndean foothills rivers flowing through the {M}adidi {N}ational {P}ark in the {B}olivian {A}mazonia. {T}he knowledge of its ichthyological diversity is, however, essential for the management and protection of aquatic ecosystems, which are threatened by the development of infrastructures (dams, factories and cities), mining and deforestation. {E}nvironmental {DNA} (e{DNA}) has been relatively little used so far in the {A}mazon basin. {W}e sampled e{DNA} from water in 34 sites in lakes and rivers in the {B}eni basin including 22 sites in the {T}uichi sub-basin, during the dry season. {T}o assess the biogeographical patterns of the amazonian ichthyofauna, we implemented a metabarcoding approach using two pairs of specific primers designed and developed in our laboratory to amplify two partially overlapping {CO}1 fragments, one of 185bp and another of 285bp. {W}e detected 252 fish taxa (207 at species level) among which 57 are newly identified for the {B}eni watershed. {S}pecies compositions are significantly different between lakes and rivers but also between rivers according to their hydrographic rank and altitude. {F}urthermore, the diversity patterns are related to the different hydro-ecoregions through which the {T}uichi flows. {T}he e{DNA} approach makes it possible to identify and complete the inventory of the ichthyofauna in this still poorly documented {A}mazon basin. {H}owever, taxonomic identification remains constrained by the lack of reference barcodes in public databases and does not allow the assignment of all {OTU}s. {O}ur results can be taken into account in conservation and management strategies and could serve as a baseline for future studies, including on other {A}ndean tributaries.},
  keywords = {{BOLIVIE} ; {AMAZONIE} ; {ANDES} ; {ZONE} {TROPICALE} ; {MADIDI} {PARC} {NATIONAL} ; {AMAZONE} {BASSIN} ; {TUICHI} {BASSIN} ; {BENI} {BASSIN}},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {17},
  numero = {1},
  pages = {e0262357 [25 ]},
  ISSN = {1932-6203},
  year = {2022},
  DOI = {10.1371/journal.pone.0262357},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085913},
}