Hongjaisee S. auteur aut IRD Jabjainai Y. auteur aut IRD Sakset S. auteur aut IRD Preechasuth K. auteur aut IRD Ngo-Giang-Huong Nicole auteur aut IRD Khamduang W. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Nucleic acid extraction from biological samples is an important step for hepatitis C virus (HCV) diagnosis. However, such extractions are mostly based on silica-based column methodologies, which may limit their application for on-site diagnosis. A simple, rapid, and field-deployable method for RNA extraction is still needed. In this study, we evaluated the efficacy of four simple RNA extraction methods for the detection of HCV in plasma samples: a silica-membrane-based method, a magnetic-beads-based method, boiling with diethyl pyrocarbonate (DEPC)-treated distilled water, and using a commercial lysis buffer. HCV RNA was detected using both real-time reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Using real-time RT-PCR, extracted RNA from the silica-membrane-based and magnetic-beads-based methods had a 100% detection rate for RNA extraction from plasma. Using RT-LAMP, extracted RNA from the silica-membrane-based method showed a 66% detection rate, while the magnetic-beads-based method had a 62% detection rate. In summary, magnetic-beads-based extraction can be used as an alternative RNA extraction method for on-site HCV detection. Boiling with DEPC-treated distilled water was not appropriate for low HCV load samples, and boiling with a lysis buffer was not recommended. specialized RNA extraction HCV molecular diagnosis RT-LAMP RT-PCR plasma 052 050 12 7 1599 [6 p.] 2022 https://www.documentation.ird.fr/hor/fdi:010085899 10.3390/diagnostics12071599 [F B010085899] https://www.documentation.ird.fr/hor/fdi:010085899 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-09/010085899.pdf IRD - Base Horizon / Pleins textes 2022-09-28 2025-02-24 fdi:010085899 fre