%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Hongjaisee, S.
%A Jabjainai, Y.
%A Sakset, S.
%A Preechasuth, K.
%A Ngo-Giang-Huong, Nicole
%A Khamduang, W.
%T Comparison of simple RNA extraction methods for molecular diagnosis of hepatitis C virus in plasma
%D 2022
%L fdi:010085899
%G ENG
%J Diagnostics
%K RNA extraction ; HCV ; molecular diagnosis ; RT-LAMP ; RT-PCR ; plasma
%M ISI:000833235200001
%N 7
%P 1599 [6 ]
%R 10.3390/diagnostics12071599
%U https://www.documentation.ird.fr/hor/fdi:010085899
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-09/010085899.pdf
%V 12
%W Horizon (IRD)
%X Nucleic acid extraction from biological samples is an important step for hepatitis C virus (HCV) diagnosis. However, such extractions are mostly based on silica-based column methodologies, which may limit their application for on-site diagnosis. A simple, rapid, and field-deployable method for RNA extraction is still needed. In this study, we evaluated the efficacy of four simple RNA extraction methods for the detection of HCV in plasma samples: a silica-membrane-based method, a magnetic-beads-based method, boiling with diethyl pyrocarbonate (DEPC)-treated distilled water, and using a commercial lysis buffer. HCV RNA was detected using both real-time reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Using real-time RT-PCR, extracted RNA from the silica-membrane-based and magnetic-beads-based methods had a 100% detection rate for RNA extraction from plasma. Using RT-LAMP, extracted RNA from the silica-membrane-based method showed a 66% detection rate, while the magnetic-beads-based method had a 62% detection rate. In summary, magnetic-beads-based extraction can be used as an alternative RNA extraction method for on-site HCV detection. Boiling with DEPC-treated distilled water was not appropriate for low HCV load samples, and boiling with a lysis buffer was not recommended.
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