@article{fdi:010085899,
  title = {{C}omparison of simple {RNA} extraction methods for molecular diagnosis of hepatitis {C} virus in plasma},
  author = {{H}ongjaisee, {S}. and {J}abjainai, {Y}. and {S}akset, {S}. and {P}reechasuth, {K}. and {N}go-{G}iang-{H}uong, {N}icole and {K}hamduang, {W}.},
  editor = {},
  language = {{ENG}},
  abstract = {{N}ucleic acid extraction from biological samples is an important step for hepatitis {C} virus ({HCV}) diagnosis. {H}owever, such extractions are mostly based on silica-based column methodologies, which may limit their application for on-site diagnosis. {A} simple, rapid, and field-deployable method for {RNA} extraction is still needed. {I}n this study, we evaluated the efficacy of four simple {RNA} extraction methods for the detection of {HCV} in plasma samples: a silica-membrane-based method, a magnetic-beads-based method, boiling with diethyl pyrocarbonate ({DEPC})-treated distilled water, and using a commercial lysis buffer. {HCV} {RNA} was detected using both real-time reverse transcription polymerase chain reaction ({RT}-{PCR}) and reverse transcription loop-mediated isothermal amplification ({RT}-{LAMP}). {U}sing real-time {RT}-{PCR}, extracted {RNA} from the silica-membrane-based and magnetic-beads-based methods had a 100% detection rate for {RNA} extraction from plasma. {U}sing {RT}-{LAMP}, extracted {RNA} from the silica-membrane-based method showed a 66% detection rate, while the magnetic-beads-based method had a 62% detection rate. {I}n summary, magnetic-beads-based extraction can be used as an alternative {RNA} extraction method for on-site {HCV} detection. {B}oiling with {DEPC}-treated distilled water was not appropriate for low {HCV} load samples, and boiling with a lysis buffer was not recommended.},
  keywords = {{RNA} extraction ; {HCV} ; molecular diagnosis ; {RT}-{LAMP} ; {RT}-{PCR} ; plasma},
  booktitle = {},
  journal = {{D}iagnostics},
  volume = {12},
  numero = {7},
  pages = {1599 [6 p.]},
  year = {2022},
  DOI = {10.3390/diagnostics12071599},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085899},
}