@article{fdi:010085885,
  title = {{S}erological and molecular detection of {L}eishmania species in dog peripheral blood from {B}obo-{D}ioulasso city, a confirmation of canine leishmaniasis enzootic area for {B}urkina {F}aso},
  author = {{D}jibougou, {A}. {D}. and {N}iki{\`e}ma, {A}. {S}. and {H}ien, {A}. {S}. and {S}angar{\'e}, {I}. and {Y}ameogo, {B}. {K}. and {K}oala, {L}. and {O}uari, {A}. and {D}iagbouga, {S}. {P}. and {D}iabat{\'e}, {A}. and {P}rice, {H}. and {F}ournet, {F}lorence and {D}abir{\'e}, {R}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}anine leishmaniasis is increasingly reported worldwide and represent a threat to both animal and human health. {I}n a previous pilot study conducted in {B}obo-{D}ioulasso, the second town of {B}urkina {F}aso, we reported five cases of canine leishmaniasis. {W}ith the perspective of a {O}ne {H}ealth action plan, and in the context of increasing urbanization, this study aimed to provide new information on {L}eishmania spp in dogs in this city. {A} cross-sectional survey was carried out from {M}ay to {A}ugust 2018 in six districts of the city in order to record clinical and biological data from domestic dogs randomly selected per district. {B}lood samples were collected into {EDTA} tubes (4-5 m{L}), treated and stored at-20 {C} until further analyses. {T}he infection status of the dogs was performed by serological tests using plasma, and real time-{PCR} ({RT}-{PCR}) to detect {L}eishmania parasites using buffy coats. {N}ested {PCR} was used for typing the {L}eishmania species in dogs which were found to be {RT}-{PCR} positive. {A} total of 147 dogs were examined clinically and sampled for blood collection, including 53.7% females and 46.3% of males with a median age of 3 years. {T}he seroincidence of {L}eishmania parasites within this dog population was 4.76% (95% {CI}:2.26-9.72). {T}he incidence of {L}eishmania was 10.88% (95% {CI}: 6.73-17.11) by {RT}-{PCR} which was significantly more sensitive (p = 0,047) and a fair concordance was observed between both tests ({K}appa = 0.39, p < 0.001). {T}he characterization of {L}eishmania species revealed that {L}. major was circulating in this domestic dog population. {O}ur results confirmed the persistence of zoonotic circulation of {L}eishmania parasites such as {L}. major currently in {B}obo-{D}ioulasso city and highlight the need for targeted interventions in order to control transmission of leishmaniasis in this region.},
  keywords = {{L}eishmania spp ; {Z}oonosis ; {D}iagnostic ; {I}ncidence ; {B}obo-{D}ioulasso ; {B}urkina {F}aso ; {BURKINA} {FASO} ; {BOBO} {DIOULASSO}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {103},
  numero = {},
  pages = {105327 [7 p.]},
  ISSN = {1567-1348},
  year = {2022},
  DOI = {10.1016/j.meegid.2022.105327},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085885},
}