@article{fdi:010085873,
  title = {{H}uman {I}g{G} responses to the {A}edes albopictus 34k2 salivary protein : analyses in {R}eunion {I}sland and {B}olivia confirm its suitability as marker of host exposure to the tiger mosquito},
  author = {{M}ontero, {S}. {B}. and {G}abrieli, {P}. and {P}oinsignon, {A}nne and {Z}amble, {B}. {Z}. {H}. and {L}ombardo, {F}. and {R}emou{\'e}, {F}ranck and {A}rca, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he rapid worldwide spreading of {A}edes aegypti and {A}edes albopictus is expanding the risk of arboviral diseases transmission, pointing out the urgent need to improve monitoring and control of mosquito vector populations. {A}ssessment of human-vector contact, currently estimated by classical entomological methods, is crucial to guide planning and implementation of control measures and evaluate transmission risk. {A}ntibody responses to mosquito genus-specific salivary proteins are emerging as a convenient complementary tool for assessing host exposure to vectors. {W}e previously showed that {I}g{G} responses to the {A}e. albopictus 34k2 salivary protein (al34k2) allow detection of seasonal and geographic variation of human exposure to the tiger mosquito in two temperate areas of {N}ortheast {I}taly. {T}he main aim of this study was to confirm and extend these promising findings to tropical areas with ongoing arboviral transmission. {M}ethods: {I}g{G} responses to al34k2 and to the {A}e. aegypti orthologous protein ae34k2 were measured by {ELISA} in cohorts of subjects only exposed to {A}e. albopictus ({R}eunion {I}sland), only exposed to {A}e. aegypti ({B}olivia) or unexposed to both these vectors ({N}orth of {F}rance). {R}esults and conclusion: {A}nti-al34k2 {I}g{G} levels were significantly higher in sera of individuals from {R}eunion {I}sland than in unexposed controls, indicating that al34k2 may be a convenient and reliable proxy for whole saliva or salivary gland extracts as an indicator of human exposure to {A}e. albopictus. {B}olivian subjects, exposed to bites of {A}e. aegypti, carried in their sera {I}g{G} recognizing the {A}e. albopictus al34k2 protein, suggesting that this salivary antigen can also detect, even though with low sensitivity, human exposure to {A}e. aegypti. {O}n the contrary, due to the high background observed in unexposed controls, the recombinant ae34k2 appeared not suitable for the evaluation of human exposure to {A}edes mosquitoes. {O}verall, this study confirmed the suitability of anti-al34k2 {I}g{G} responses as a specific biomarker of human exposure to {A}e. albopictus and, to a certain extent, to {A}e. aegypti. {I}mmunoassays based on al34k2 are expected to be especially effective in areas where {A}e. albopictus is the main arboviral vector but may also be useful in areas where {A}e. albopictus and {A}e. aegypti coexist.},
  keywords = {{A}edes albopictus ; {A}edes aegypti ; 34k2 salivary protein ; {H}ost exposure ; {S}erological marker ; {V}ector control ; {H}uman-vector contact ; {REUNION} ; {BOLIVIE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {15},
  numero = {1},
  pages = {260 [8 ]},
  ISSN = {1756-3305},
  year = {2022},
  DOI = {10.1186/s13071-022-05383-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085873},
}