@article{fdi:010085713,
  title = {{VNTR} typing of the bacterial rice pathogen {B}urkholderia glumae reveals the coexistence of several diverging lineages in a single field in {C}olombia},
  author = {{B}{\'e}na, {G}illes and {F}ory, {P}. {A}. and {R}ico, {J}. {E}. and {M}osquera, {G}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}urkholderia glumae is responsible for the panicle blight disease of rice. {T}his disease is present worldwide and can result in significant drop in yields. {T}o estimate the genetic diversity of the bacterial strains present in a rice paddy field in {C}olombia, we sampled 109 strains from infected panicles. {T}o detect fine genetic relationships among related haplotypes, and to overcome a very low nucleotide diversity detected in previous studies, we designed primers to amplify and sequence several highly variable minisatellite loci, or variable number tandem repeats ({VNTR}s), as well as part of the {T}oxoflavin tox{A} gene in all strains. {R}esults show that the tox{A} nucleotide diversity defined four lineages and was similar to that detected in several fields in {J}apan; data suggest that {B}. glumae has spread from {A}sia to {A}merica without major loss of genetic diversity, and that five {VNTR} loci discriminated the strains within the field revealing single and multi-infections of the rice panicles with a wide distribution of the haplotypes among the different plots. {E}ven though disease levels vary considerably from year to year, the bacterial genetic diversity is maintained within a field. {W}e do not detect any geographical structuring within the field, nor any effect of the rice cultivar on the observed diversity. {T}he consequences on the origin and evolution of the bacteria are discussed.},
  keywords = {{B}urkholderia glumae ; {C}olombia ; population structure ; rice ; {VNTR} ; {COLOMBIE}},
  booktitle = {},
  journal = {{P}lant {D}isease},
  volume = {105},
  numero = {11},
  pages = {3466--3473},
  ISSN = {0191-2917},
  year = {2021},
  DOI = {10.1094/pdis-02-21-0235-re},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085713},
}