Lerner A. auteur aut IRD Herschkovitz Y. auteur aut IRD Baudoin Ezékiel auteur aut IRD Nazaret S. auteur aut IRD Moënne-Loccoz Y. auteur aut IRD Okon Y. auteur aut IRD Jurkevitch E. auteur aut IRD text journalArticle eng text/pdf born digital access Nucleic acid-based techniques allow the exploration of microbial communities in the environments such as the rhizosphere. Azospirillum brasilense, a plant growth promoting rhizobacterium (PGPR), causes morphological changes in the plant root system. These changes in root physiology may indirectly affect the microbial diversity of the rhizosphere. In this study, the changes in the rhizobacterial structure following A. brasilense inoculation of maize (Zea mays) plants was examined by PCR-denaturating gradient gel electrophoresis (DGGE) and automated ribosomal intergenic spacer analysis (ARISA), using two universal primers sets for the 16S rRNA gene, and an intergenic 16S-23S rDNA primer set, respectively. Similar results were obtained when using either ARISA or DGGE performed with these different primer sets, and analyzed by different statistical methods: no prominent effect of A. brasilense inoculation was observed on the bacterial communities of plant roots grown in two different soils and in different growth systems. In contrast, plant age caused significant shifts in the bacterial populations. specialized FRANCE 076 084 074 021 38 6 1212-1218 2006 0038-0717 https://www.documentation.ird.fr/hor/fdi:010085707 10.1016/j.soilbio.2005.10.007 0038-0717 [F B010085707] https://www.documentation.ird.fr/hor/fdi:010085707 https://www.documentation.ird.fr/intranet/publi/2023-01/010085707.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2022-07-20 2023-01-11 fdi:010085707 fre