@inproceedings{fdi:010085568,
  title = {{M}icrosatellite markers in the entomopathogenic fungus {P}aecilomyces fumosoroseus for monitoring of isolates introduced against {B}emisia tabaci, epidemiological and population genetics studies [r{\'e}sum{\'e}]},
  author = {{G}authier, {N}athalie and {B}on, {M}arie-{C}laude and {D}alleau-{C}louet, {C}{\'e}cile and {F}argues, {J}acques},
  editor = {},
  language = {{ENG}},
  abstract = {{O}ne of the greatest challenges for improving the use of fungi, not only as microbial control agents but also for all epidemiological and population genetics studies, is the ablity to identify the isolates used and analyzed. {T}he haploid entomopathogenic hyphomycete {P}aecilomyces fumosoroseus ({P}fr) used for biological control of economically serious agricultural pests is a geographically widespread fungus infecting various orders of insects. {I}n particular, it is naturally associated with the whitefly {B}emisia tabaci ({H}omoptera {A}leyrodidae) in various geoclimatic regions. {O}ngoing difficulties in applying morphological approaches to isolate-recognition {P}fr have spurred the search for discriminant markers. {B}ased on r{DNA}-{ITS} {RFLP} approach, all isolates collected from {B}. tabaci clustered in 1 group although they originated from diverse geographical locations. {M}icrosatellite markers were therefore developed and characterized in {P}fr. {U}pon the 9 isolated, 8 were revealed as polymorphic. {A}mplification success and length and sequence variability of these markers were investigated on a set of 34 {P}fr sampled from various insect-host species and geographical locations. {F}irst, {PCR} amplifications at the 9 previously designed loci were only successful with {P}fr from {H}omoptera hosts (25 {B}. tabaci and 1 {P}henacoccus sp. hosts) which has suggested existence of genotype host-associations. {S}econd, allele size and above all sequence analyses (flanking microsatellite-regions and/or microsatellite regions) improve our ability to discriminatamong {P}fr isolates and provide a means to subdivide isolates obtained from {B}. tabaci. {I}ndeed, from a unique {P}fr r{DNA}-{ITS} {RFLP} haplotype, 7 patterns based on specific microsatellite allelic-sizes and 14 haplotypes based on their sequencing were resolved from {B}. tabaci which emphasizes their value for further epidemiological studies and use in biological control. {F}inally, their value for genetic relationships inference was also proven and a phylogeographic framework was henceforth established providing evidence for two distinct lineages of {P}fr, {A}merican-{C}uban and {A}sian, with a putative {I}ndian origin of the {A}merican-{C}uban {P}fr group from an {I}ndian group. {C}onsequently, the microsatellite markers described represent a potent tool for {P}fr population diversity studies and open a new and informative window on the use of well-discriminated isolates in epidemiology and biological control strategy against {B}. tabaci.},
  keywords = {},
  volume = {8},
  numero = {4},
  pages = {19},
  booktitle = {},
  year = {2008},
  DOI = {10.1673/031.008.0401},
  ISSN = {1536-2442},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085568},
}