%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gonin, M.
%A Jeong, Kwanho
%A Coudert, Y.
%A Lavarenne, J.
%A Hoang, G. T.
%A Bes, M.
%A To, H. T. M.
%A Thiaw, M. R. N.
%A Do, T. V.
%A Moukouanga, Daniel
%A Guyomarc'h, S.
%A Bellande, Kevin
%A Brossier, J. R.
%A Parizot, B.
%A Nguyen, H. T.
%A Beeckman, T.
%A Bergougnoux, V.
%A Rouster, J.
%A Sallaud, C.
%A Laplaze, Laurent
%A Champion, Antony
%A Gantet, Pascal
%T CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in rice
%D 2022
%L fdi:010085414
%G ENG
%J Plant Journal
%@ 0960-7412
%K ASL ; LBD transcription factor ; CRL1 ; DNA binding domain ; crown root ; development ; rice ; gene regulatory network ; Oryza sativa
%M ISI:000825771600001
%P [21 ]
%R 10.1111/tpj.15838
%U https://www.documentation.ird.fr/hor/fdi:010085414
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-09/010085414.pdf
%V [Early access]
%W Horizon (IRD)
%X In cereals, the root system is mainly composed of post-embryonic shoot-borne roots, named crown roots. The CROWN ROOTLESS1 (CRL1) transcription factor, belonging to the ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) family, is a key regulator of crown root initiation in rice (Oryza sativa). Here, we show that CRL1 can bind, both in vitro and in vivo, not only the LBD-box, a DNA sequence recognized by several ASL/LBD transcription factors, but also another not previously identified DNA motif that was named CRL1-box. Using rice protoplast transient transactivation assays and a set of previously identified CRL1-regulated genes, we confirm that CRL1 transactivates these genes if they possess at least a CRL1-box or an LBD-box in their promoters. In planta, ChIP-qPCR experiments targeting two of these genes that include both a CRL1- and an LBD-box in their promoter show that CRL1 binds preferentially to the LBD-box in these promoter contexts. CRISPR/Cas9-targeted mutation of these two CRL1-regulated genes, which encode a plant Rho GTPase (OsROP) and a basic helix-loop-helix transcription factor (OsbHLH044), show that both promote crown root development. Finally, we show that OsbHLH044 represses a regulatory module, uncovering how CRL1 regulates specific processes during crown root formation.
%$ 076