@article{fdi:010085414,
  title = {{CROWN} {ROOTLESS}1 binds {DNA} with a relaxed specificity and activates {O}s{ROP} and {O}sb{HLH}044 genes involved in crown root formation in rice},
  author = {{G}onin, {M}. and {J}eong, {K}wanho and {C}oudert, {Y}. and {L}avarenne, {J}. and {H}oang, {G}. {T}. and {B}es, {M}. and {T}o, {H}. {T}. {M}. and {T}hiaw, {M}. {R}. {N}. and {D}o, {T}. {V}. and {M}oukouanga, {D}aniel and {G}uyomarc'h, {S}. and {B}ellande, {K}evin and {B}rossier, {J}. {R}. and {P}arizot, {B}. and {N}guyen, {H}. {T}. and {B}eeckman, {T}. and {B}ergougnoux, {V}. and {R}ouster, {J}. and {S}allaud, {C}. and {L}aplaze, {L}aurent and {C}hampion, {A}ntony and {G}antet, {P}ascal},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n cereals, the root system is mainly composed of post-embryonic shoot-borne roots, named crown roots. {T}he {CROWN} {ROOTLESS}1 ({CRL}1) transcription factor, belonging to the {ASYMMETRIC} {LEAVES}2-{LIKE}/{LATERAL} {ORGAN} {BOUNDARIES} {DOMAIN} ({ASL}/{LBD}) family, is a key regulator of crown root initiation in rice ({O}ryza sativa). {H}ere, we show that {CRL}1 can bind, both in vitro and in vivo, not only the {LBD}-box, a {DNA} sequence recognized by several {ASL}/{LBD} transcription factors, but also another not previously identified {DNA} motif that was named {CRL}1-box. {U}sing rice protoplast transient transactivation assays and a set of previously identified {CRL}1-regulated genes, we confirm that {CRL}1 transactivates these genes if they possess at least a {CRL}1-box or an {LBD}-box in their promoters. {I}n planta, {C}h{IP}-q{PCR} experiments targeting two of these genes that include both a {CRL}1- and an {LBD}-box in their promoter show that {CRL}1 binds preferentially to the {LBD}-box in these promoter contexts. {CRISPR}/{C}as9-targeted mutation of these two {CRL}1-regulated genes, which encode a plant {R}ho {GTP}ase ({O}s{ROP}) and a basic helix-loop-helix transcription factor ({O}sb{HLH}044), show that both promote crown root development. {F}inally, we show that {O}sb{HLH}044 represses a regulatory module, uncovering how {CRL}1 regulates specific processes during crown root formation.},
  keywords = {{ASL} ; {LBD} transcription factor ; {CRL}1 ; {DNA} binding domain ; crown root ; development ; rice ; gene regulatory network ; {O}ryza sativa},
  booktitle = {},
  journal = {{P}lant {J}ournal},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[21 p.]},
  ISSN = {0960-7412},
  year = {2022},
  DOI = {10.1111/tpj.15838},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085414},
}