@article{fdi:010085387,
  title = {{D}ifferential genetic strategies of {B}urkholderia vietnamiensis and {P}araburkholderia kururiensis for root colonization of {O}ryza sativa subsp. japonica and {O}. sativa subsp. indica, as revealed by transposon mutagenesis sequencing},
  author = {{W}allner, {A}. and {B}usset, {N}icolas and {L}achat, {J}. and {G}uigard, {L}. and {K}ing, {E}. and {R}imbault, {I}sabelle and {M}ergaert, {P}. and {B}{\'e}na, {G}illes and {M}oulin, {L}ionel},
  editor = {},
  language = {{ENG}},
  abstract = {{B}urkholderiaceae are frequent and abundant colonizers of the rice rhizosphere and interesting candidates to investigate for growth promotion. {S}pecies of {P}araburkholderia have repeatedly been described to stimulate plant growth. {B}urkholderia vietnamiensis {LMG}10929 and {P}araburkholderia kururiensis {M}130 are bacterial rice growth-promoting models. {B}esides this common ecological niche, species of the {B}urkholderia genus are also found as opportunistic human pathogens, while {P}araburkholderia species are mostly environmental and plant associated. {I}n this study, we compared the genetic strategies used by {B}. vietnamiensis and {P}. kururiensis to colonize two subspecies of their common host, {O}ryza sativa subsp. japonica (cv. {N}ipponbare) and {O}. sativa subsp. indica (cv. {IR}64). {W}e used high-throughput screening of transposon insertional mutant libraries ({T}n-seq) to infer which genetic elements have the highest fitness contribution during root surface colonization at 7 days postinoculation. {O}verall, we detected twice more genes in {B}. vietnamiensis involved in rice root colonization than in {P}. kururiensis, including genes contributing to the tolerance of plant defenses, which suggests a stronger adverse reaction of rice toward {B}. vietnamiensis than toward {P}. kururiensis. {F}or both strains, the bacterial fitness depends on a higher number of genes when colonizing indica rice compared to japonica. {T}hese divergences in host pressure on bacterial adaptation could be partly linked to the cultivars' differences in nitrogen assimilation. {W}e detected several functions commonly enhancing root colonization in both bacterial strains, e.g., {E}ntner-{D}oudoroff ({ED}) glycolysis. {L}ess frequently and more strain specifically, we detected functions limiting root colonization such as biofilm production in {B}. vietnamiensis and quorum sensing in {P}. kururiensis. {T}he involvement of genes identified through the {T}n-seq procedure as contributing to root colonization, i.e., {ED} pathway, c-di-{GMP} cycling, and cobalamin synthesis, was validated by directed mutagenesis and competition with wild-type ({WT}) strains in rice root colonization assays. {IMPORTANCE} {B}urkholderiaceae are frequent and abundant colonizers of the rice rhizosphere and interesting candidates to investigate for growth promotion. {S}pecies of {P}araburkholderia have repeatedly been described to stimulate plant growth. {H}owever, the closely related {B}urkholderia genus includes both beneficial and phytopathogenic species, as well as species able to colonize animal hosts and cause disease in humans. {W}e need to understand to what extent the bacterial strategies used for the different biotic interactions differ depending on the host and if strains with agricultural potential could also pose a threat toward other plant hosts or humans. {T}o start answering these questions, we used in this study transposon sequencing to identify genetic traits in {B}urkholderia vietnamiensis and {P}araburkholderia kururiensis that contribute to the colonization of two different rice varieties. {O}ur results revealed large differences in the fitness gene sets between the two strains and between the host plants, suggesting a strong specificity in each bacterium-plant interaction.},
  keywords = {{P}araburkholderia kururiensis ; {B}urkholderia vietnamiensis ; root ; colonization ; {T}n-seq ; {PGPR} ; rice},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {22},
  numero = {},
  pages = {100523 [18 ]},
  ISSN = {0099-2240},
  year = {2022},
  DOI = {10.1128/aem.00642-22},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085387},
}