@article{fdi:010085371,
  title = {{I}dentification of ticks from an old collection by {MALDI}-{TOF} {MS}},
  author = {{M}'{M}adi, {S}. {A}. and {D}iarra, {A}dama {Z}an and {A}lmeras, {L}. and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective of this study is to find the optimal conditions for preparing the samples, resulting in quality, reproducible and specific {MS} spectra of the ticks, with a shelf life in 70% ethanol of more than ten years. {A}mblyomma ({A}m.) variegatum species which had been stored in alcohol for more than twenty years and for which numerous specimens were available were used to compare the performance of four protocols tested. {S}pectra of insufficient quality were obtained from {A}m. variegatum legs preserved in alcohol for long periods with the reference protocol, named {DO} that we had set up years ago. {T}he same observation was made on the spectra from {A}m. variegatum legs from dry (evaporated alcohol, {DO}-mod protocol). {W}ith new protocols named {R}e{DO} and {PR}e{DO} the spectra were of good quality with high intensities (> 3000 a.u.). {B}lind testing showed that 94%, and 93% of the spectra were correctly identified with relevant log score values ({LSV}s >= 1.8), respectively for {R}e{DO} and {PR}e{DO} protocols. {A}ll soft ticks treated in this study by {PR}e{DO} protocol exhibited low intensity spectra with background noise. {T}his study revealed that {MALDI}-{TOF} {MS} is able to identify hard ticks stored during decades in alcohol or dry (evaporated alcohol). {S}ignificance of the study: {T}he correct identification of ticks, including vectors responsible for the transmission of infectious diseases in humans and animals is essential for their control. {MALDI}-{TOF} {MS}, a proteomic tool that has emerged in recent years, has become an innovative, accurate and alternative tool for the identification of arthropods, including ticks. {H}owever, previous studies reported that preservation of arthropods in alcohol modified the {MS} spectra obtained from specimens of the same species freshly collected or frozenly stored. {I}n this study, a standard protocol was established for the identification of tick collections which had been stored for more than ten years in alcohol. {F}our different protocols were assessed. {T}he analysis of the results showed that among the four protocols tested, two protocols named {R}e{DO} ({R}ehydration and incubation of the legs in 40 mu l of {HPLC} water for 12 h in a dry bath at 37 degrees) and {P}re{DO} ({D}rying of the legs for 12 h in a dry bath at 37 degrees {C} followed by rehydration and incubation in 40 mu l of {HPLC} water for 12 h.) seem to be more appropriate for the {MALDI}-{TOF} {MS} identification of ticks from old collections preserved in alcohol or dry. {T}his study is promising for the future, as it will make it possible to create a {MALDI}-{TOF} {MS} database from a wide range of ticks which have been stored for a long time in alcohol or which are dry stored in laboratories and museums around the world.},
  keywords = {{T}ick ; {A}lcohol preservation ; {MALDI}-{TOF} {MS} ; {E}ntomology},
  booktitle = {},
  journal = {{J}ournal of {P}roteomics},
  volume = {264},
  numero = {},
  pages = {104623 [13 ]},
  ISSN = {1874-3919},
  year = {2022},
  DOI = {10.1016/j.jprot.2022.104623},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085371},
}