Abdulla H. H. A. M. auteur aut IRD Aboelsoued D. auteur aut IRD Fang T. K. auteur aut IRD Abdel-Shaft S. auteur aut IRD Megeed K. N. A. auteur aut IRD Parola P. auteur aut IRD Raoult D. auteur aut IRD Mediannikov Oleg auteur aut IRD text journalArticle eng text/pdf born digital access Equine vector-borne diseases (EVBDs) are emerging and re-emerging diseases, and most of them are zoonotic. This study aimed to investigate EVBDs in equines and associated arthropods (ticks and flies) from Egypt using molecular analyses, in addition to a preliminary characterization of associated ticks and flies by the matrixassisted laser desorption/ionization time of flight (MALDI-TOF) and molecular techniques. In this study, 335 blood samples were obtained from equines that appeared to be in good health (320 horses and 15 donkeys) in Cairo and Beni Suef provinces, Egypt. From the same animals, 166 arthropods (105 sucking flies and 61 ticks) were collected. Ticks and flies were preliminary characterized by the MALDI-TOF and molecular tools. Quantitative PCR (qPCR) and standard PCR coupled with sequencing were performed on the DNA of equines, ticks, and flies to screen multiple pathogens. The MALDI-TOF and molecular characterization of arthropods revealed that louse fly (Hippobosca equina) and cattle tick (Rhipicephalus annulatus) infesting equines. Anaplasma platys-like (1.6%), Anaplasma marginale (1.6%), Candidatus Ehrlichia rustica (6.6%), a new Ehrlichia sp. (4.9%), and Borrelia theileri (3.3%) were identified in R. annulatus. Anaplasma sp. and Borrelia sp. DNAs were only detected in H. equina by qPCR. A. marginale, Anaplasma ovis, and Theileria ovis recorded the same low infection rate (0.6%) in donkeys, while horses were found to be infected with Theileria equi and a new Theileria sp. Africa with recorded prevalence rates of 1.2% and 2.7%, respectively. In conclusion, different pathogens were first detected such as A. platys-like, Candidatus E. rustica, and a new Ehrlichia sp. in R. annulatus; A. marginale, A. ovis, and T. ovis in donkeys; and a new Theileria sp. "Africa" in horses. specialized Equine Hippobosca equina MALDI-TOF PCR Rhipicephalus annulatus Vector-borne diseases EGYPTE 052 080 227 106274 [13 ] 2022 0001-706X https://www.documentation.ird.fr/hor/fdi:010085369 10.1016/j.actatropica.2021.106274 0001-706X [F B010085369] https://www.documentation.ird.fr/hor/fdi:010085369 https://www.documentation.ird.fr/intranet/publi/2022-09/010085369.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2022-09-02 2025-02-24 fdi:010085369 fre