@article{fdi:010085363,
  title = {{E}nhanced procedures for mosquito identification by {MALDI}-{TOF} {MS}},
  author = {{B}amou, {R}. and {C}osta, {M}. {M}. and {D}iarra, {A}dama {Z}an and {M}artins, {A}. {J}. and {P}arola, {P}. and {A}lmeras, {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {I}n the last decade, an innovative approach has emerged for arthropod identification based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}). {I}ncreasing interest in applying the original technique for arthropod identification has led to the development of a variety of procedures for sample preparation and selection of body parts, among others. {H}owever, the absence of a consensual strategy hampers direct inter-study comparisons. {M}oreover, these different procedures are confusing to new users. {E}stablishing optimized procedures and standardized protocols for mosquito identification by {MALDI}-{TOF} {MS} is therefore a necessity, and would notably enable the sharing of reference {MS} databases. {H}ere, we assess the optimal conditions for mosquito identification using {MALDI}-{TOF} {MS} profiling. {M}ethods {T}hree homogenization methods, two of which were manual and one automatic, were used on three distinct body parts (legs, thorax, head) of two mosquito laboratory strains, {A}nopheles coluzzii and {A}edes aegypti, and the results evaluated. {T}he reproducibility of {MS} profiles, identification rate with relevant scores and the suitability of procedures for high-throughput analyses were the main criteria for establishing optimized guidelines. {A}dditionally, the consequences of blood-feeding and geographical origin were evaluated using both laboratory strains and field-collected mosquitoes. {R}esults {R}elevant score values for mosquito identification were obtained for all the three body parts assayed using {MALDI}-{TOF} {MS} profiling; however, the thorax and legs were the most suitable specimens, independently of homogenization method or species. {A}lthough the manual homogenization methods were associated with a high rate of identification on the three body parts, this homogenization mode is not adaptable to the processing of a large number of samples. {T}herefore, the automatic homogenization procedure was selected as the reference homogenization method. {B}lood-feeding status did not hamper the identification of mosquito species, despite the presence of {MS} peaks from original blood in the {MS} profiles of the three body parts tested from both species. {F}inally, a significant improvement in identification scores was obtained for field-collected specimens when {MS} spectra of species from the same geographical area were added to the database. {C}onclusion {T}he results of the current study establish guidelines for the selection of mosquito anatomic parts and modality of sample preparation (e.g. homogenization) for future specimen identification by {MALDI}-{TOF} {MS} profiling. {T}hese standardized operational protocols could be used as references for creating an international {MS} database.},
  keywords = {{M}osquitoes identification ; {MALDI}-{TOF} {MS} ; {O}ptimization ; {S}tandardization},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {15},
  numero = {1},
  pages = {240 [17 p.]},
  ISSN = {1756-3305},
  year = {2022},
  DOI = {10.1186/s13071-022-05361-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085363},
}