@article{fdi:010085350,
  title = {{I}mmuno-enzymatic and proteomic approaches for sexing the {A}frican {B}onytongue ({H}eterotis niloticus {C}uvier, 1829)},
  author = {{K}oua, {N}. {D}. and {H}enry, {J}. and {C}orre, {E}. and {P}ontin, {J}. and {B}ernay, {B}. and {N}unez {R}odriguez, {J}esus},
  editor = {},
  language = {{ENG}},
  abstract = {{H}eterotis niloticus is an {A}frican species of {O}steoglossiformes that presents biological peculiarities and zootechnical performances favorable for fish farming. {H}owever, the absence of a sexual dimorphism hinders the optimization of its reproduction in captivity and limits the understanding of its reproductive behavior. {T}his study is aimed at developing a minimally invasive and reliable sexing method to detect vitellogenin ({V}tg) in female plasma. {A} commercial sexing kit ({A}cobium, {M}ontpellier, {F}rance) for {A}rapaima gigas-a phylogenetically sister species of {H}. niloticus-successfully identified only 20% of mature {H}. niloticus females. {E}nzyme-linked immunosorbent assays ({ELISA}) were carried out using three {V}tg antibodies. {T}he {A}. gigas {V}tg1 antibody cross-reacted significantly with plasma dilutions of female {H}. niloticus ranging from 1:1000 to 1:10,000, but with relatively low intensity. {T}he {V}tg antibody from {O}steoglossum bicirrhosum, another species of {O}steoglossiformes, showed non-specific binding with the {V}tg of {H}. niloticus female plasma. {F}inally, an antibody for {H}. niloticus {V}tg developed in this study allowed us to differentiate the two sexes with plasma coating dilutions ranging from 1:1000 to 1:10,000. {T}he results of the assay were validated by a proteomic approach showing that {V}tg-targeted mass spectrometry analysis of {H}. niloticus blood protein extracts could be used to accurately determine the presence of {V}tg in the plasma of mature females. {T}he final validation of the {ELISA} technique using the {H}. niloticus {V}tg antibody was confirmed by visual sexing of a significant number of blood-sampled fish gonads; 100% of the fish were correctly sexed by the {ELISA} method.},
  keywords = {{H}eterotis niloticus ; vitellogenin ; antibodies ; {ELISA} ; proteomics ; sexing ; {AFRIQUE} {SUBSAHARIENNE} ; {FRANCE}},
  booktitle = {},
  journal = {{F}ishes},
  volume = {7},
  numero = {3},
  pages = {106 [14 ]},
  year = {2022},
  DOI = {10.3390/fishes7030106},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085350},
}