@article{fdi:010085167,
  title = {{M}olecular and {MALDI}-{TOF} {MS} characterisation of {H}yalomma aegyptium ticks collected from turtles and their associated microorganisms in {A}lgeria},
  author = {{B}enyahia, {H}. and {D}iarra, {A}dama {Z}an and {G}herissi, {D}. {E}. and {B}erenger, {J}. {M}. and {B}enakhla, {A}. and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he identification of ticks and their associated pathogens is important for knowledge on tick-borne diseases. {T}he objective of this study was to use morphological, matrix-assisted laser desorption ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}) and/or molecular biology tools to identify ticks collected from turtles in northeastern {A}lgeria, as well as to investigate the microorganisms associated with these ticks. {A} total of 471 adult ticks were collected and identified morphologically as {H}yalomma aegyptium, of which 248 (52.7%) were female and 223 (47.3%) were male. amongst them, 230 specimens were randomly selected for molecular and {MALDI}-{TOF} {MS} analysis. {M}olecular biology confirmed that our ticks were {H}y. aegyptium. {MALDI}-{TOF} {MS} analysis revealed that 100% of the spectra were of excellent quality. {F}our spectra were selected to update our own database {MALDI}-{TOF} {MS} arthropod. {T}he blind test of the 226 remaining spectra showed that all ticks were correctly identified, with scores ranging from 1.774 to 2.655 with a mean of 2.271 +/- 0.16 of which, 223 (98.6%) had log score value ({LSV})>1.8. {M}olecular biology screening showed that the ticks carried the {DNA} of {B}orrelia turcica, {R}ickettsia africae, {R}ickettsia aeschlimannii, {R}ickettsia sibirica mongolitimonae and with the {A}naplasmataceae were close to a potentially new, undescribed {E}hrlichia sp. {T}his study confirms that {MALDI}-{TOF} {MS} is a reliable tool for the identification of ticks and that ticks collected from turtles in {A}lgeria are carriers of several species of microorganisms which may be responsible for diseases in humans and animals.},
  keywords = {{H}yalomma aegyptium ; {MALDI}-{TOF} {MS} ; {M}olecular biology ; {M}icroorganisms ; {A}lgeria ; {ALGERIE}},
  booktitle = {},
  journal = {{T}icks and {T}ick-{B}orne {D}iseases},
  volume = {13},
  numero = {1},
  pages = {101858 [10 p.]},
  ISSN = {1877-959{X}},
  year = {2022},
  DOI = {10.1016/j.ttbdis.2021.101858},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085167},
}