@article{fdi:010085151,
  title = {{I}mmunoglobulin {M} seroneutralization for improved confirmation of {J}apanese encephalitis virus infection in a flavivirus-endemic area},
  author = {{B}harucha, {T}. and {A}yhan, {N}. and {P}astorino, {B}. and {R}attanavong, {S}. and {V}ongsouvath, {M}. and {M}ayxay, {M}. and {C}hangthongthip, {A}. and {S}engvilaipaseuth, {O}. and {P}honemixay, {O}. and {P}ommier, {J}. {D}. and {G}orman, {C}. and {Z}itzmann, {N}. and {N}ewton, {P}. {N}. and {L}amballerie de, {X}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he mainstay of diagnostic confirmation of acute {J}apanese encephalitis ({JE}) involves detection of anti-{JE} virus ({JEV}) immunoglobulin {M} ({I}g{M}) by enzyme-linked immunosorbent assay ({ELISA}). {L}imitations in the specificity of this test are increasingly apparent with the introduction of {JEV} vaccinations and the endemicity of other cross-reactive flaviviruses. {V}irus neutralization testing ({VNT}) is considered the gold standard, but it is challenging to implement and interpret. {W}e performed a pilot study to assess {I}g{G} depletion prior to {VNT} for detection of anti-{JEV} {I}g{M} neutralizing antibodies ({I}g{M}-{VNT}) as compared with standard {VNT}. {M}ethods {W}e evaluated {I}g{M}-{VNT} in paired sera from anti-{JEV} {I}g{M} {ELISA}-positive patients ({JE} n=35) and negative controls of healthy flavivirus-naive (n=10) as well as confirmed dengue (n=12) and {Z}ika virus (n=4) patient sera. {I}g{M}-{VNT} was subsequently performed on single sera from additional {JE} patients (n=76). {R}esults {A}nti-{JEV} {I}g{G} was detectable in admission serum of 58% of {JE} patients. {T}he positive, negative and overall percentage agreement of {I}g{M}-{VNT} as compared with standard {VNT} was 100%. {A} total of 12/14 (86%) patient samples were unclassified by {VNT} and, with sufficient sample available for {I}g{G} depletion and {I}g{G} {ELISA} confirming depletion, were classified by {I}g{M}-{VNT}. {I}g{M}-{VNT} enabled {JE} case classification in 72/76 (95%) patients for whom only a single sample was available. {C}onclusions {T}he novel approach has been readily adapted for high-throughput testing of single patient samples and it holds promise for incorporation into algorithms for use in reference centres.},
  keywords = {diagnostics ; flavivirus ; {L}aos ; neglected tropical disease ; neurological ; infection ; seroneutralization ; {LAOS} ; {ZONE} {TROPICALE}},
  booktitle = {},
  journal = {{T}ransactions of the {R}oyal {S}ociety of {T}ropical {M}edicine and {H}ygiene},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[11 p.]},
  ISSN = {0035-9203},
  year = {2022},
  DOI = {10.1093/trstmh/trac036},
  URL = {https://www.documentation.ird.fr/hor/fdi:010085151},
}