@article{fdi:010085115, title = {{E}ffects of ultramafic topsoil stockpiling during mine activities on its microbial diversity and other microbiological and physicochemical characteristics}, author = {{A}mir, {H}. and {B}ordez, {L}. and {C}avaloc, {Y}. and {J}ourand, {P}hilippe and {D}ucousso, {M}. and {J}uillot, {F}arid}, editor = {}, language = {{ENG}}, abstract = {{N}owadays, ecological restoration is considered the best solution for the rehabilitation of mining-degraded areas, particularly when it concerns valuable ecosystems. {T}his is the case in {N}ew {C}aledonia, a hot spot of biodiversity, with 82% of endemic plant species in ultramafic soils. {T}he use of topsoil to restore mined areas is an important practice. {H}owever, topsoil stockpiling can reduce soil fertility. {W}e studied the evolution of different properties of two topsoils stored in 10 m height piles on mined areas, in order to characterize possible degradations. {T}he effects of storage duration, depth, and topsoil origin were analyzed, focusing mainly on bacterial and fungal diversity assessed after bar-coded pyrosequencing, and expressed by operational taxonomic units ({OTU}). {M}icrobial activity (carbon dioxyde production), arbuscular mycorrhizal fungi ({AMF}) spore numbers, bulk density and different other physicochemical characteristics were also determined. {T}he studied ultramafic soils were characterized by a relatively high microbial diversity with 45 bacterial phyla and 7 fungal phyla. {B}acterial and fungal {OTU} numbers did not vary significantly after 12 months of storage, but the community structures of these groups were changed. {AMF} diversity was significantly reduced by the storage. {M}icrobial activity, {AMF} spore numbers, soil organic carbon, total nitrogen, cation exchange capacity, and carbon to nitrogen ratio ({C}/{N}) decreased significantly starting from 3 to 9 months of topsoil storage. {I}n contrast, soil bulk density and p{H} increased, and extractable concentrations of potentially toxic metals varied only slightly. {A}ll the changes induced by topsoil stockpiling in microbial population structure, {AMF} diversity, and other biotic and abiotic traits may negatively affect soil functions and create perturbations of the reconstructed ecosystems after revegetation. {I}t is then necessary to reduce the storage of ultramafic topsoils as far as possible. {I}n all cases, the stockpiling should not exceed six months before its use in ecosystem restoration. {I}t is also recommended to reduce the height of the stockpiles, when possible, to 1 m or less to minimize the compaction and the anaerobiosis.}, keywords = {{U}ltramafic soils ; {T}opsoil ; {M}icrobial diversity ; bacteria ; fungi ; {AMF} ; {NOUVELLE} {CALEDONIE}}, booktitle = {}, journal = {{E}cological {E}ngineering}, volume = {177}, numero = {}, pages = {106563 [11 ]}, ISSN = {0925-8574}, year = {2022}, DOI = {10.1016/j.ecoleng.2022.106563}, URL = {https://www.documentation.ird.fr/hor/fdi:010085115}, }