Sangboonruang S. auteur aut IRD Semakul N. auteur aut IRD Sookkree S. auteur aut IRD Kantapan J. auteur aut IRD Ngo-Giang-Huong Nicole auteur aut IRD Khamduang W. auteur aut IRD Kongyai N. auteur aut IRD Tragoolpua K. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Herpes simplex type 2 (HSV-2) infection causes a significant life-long disease. Long-term side effects of antiviral drugs can lead to the emergence of drug resistance. Thus, propolis, a natural product derived from beehives, has been proposed to prevent or treat HSV-2 infections. Unfortunately, therapeutic applications of propolis are still limited due its poor solubility. To overcome this, a nanoparticle-based drug delivery system was employed. An ethanolic extract of propolis (EEP) was encapsulated in nanoparticles composed of poly(lactic-co-glycolic acid) and chitosan using a modified oil-in-water single emulsion by using the solvent evaporation method. The produced nanoparticles (EEP-NPs) had a spherical shape with a size of similar to 450 nm and presented satisfactory physicochemical properties, including positively charged surface (38.05 +/- 7.65 mV), high entrapment efficiency (79.89 +/- 13.92%), and sustained release profile. Moreover, EEP-NPs were less cytotoxic on Vero cells and exhibited anti-HSV-2 activity. EEP-NPs had a direct effect on the inactivation of viral particles, and also disrupted the virion entry and release from the host cells. A significant decrease in the expression levels of the HSV-2 replication-related genes (ICP4, ICP27, and gB) was also observed. Our study suggests that EEP-NPs provide a strong anti-HSV-2 activity and serve as a promising platform for the treatment of HSV-2 infections. specialized herpes simplex virus type 2 antiviral activity propolis chitosan poly(lactic-co-glycolic acid) polymeric nanoparticles 020 050 052 27 8 2560 [16 p.] 2022 https://www.documentation.ird.fr/hor/fdi:010084689 10.3390/molecules27082560 [F B010084689] https://www.documentation.ird.fr/hor/fdi:010084689 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-06/010084689.pdf IRD - Base Horizon / Pleins textes 2022-06-03 2025-02-24 fdi:010084689 fre