%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sangboonruang, S.
%A Semakul, N.
%A Sookkree, S.
%A Kantapan, J.
%A Ngo-Giang-Huong, Nicole
%A Khamduang, W.
%A Kongyai, N.
%A Tragoolpua, K.
%T Activity of propolis nanoparticles against HSV-2 : promising approach to inhibiting infection and replication
%D 2022
%L fdi:010084689
%G ENG
%J Molecules
%K herpes simplex virus type 2 ; antiviral activity ; propolis ; chitosan ; poly(lactic-co-glycolic acid) ; polymeric nanoparticles
%M ISI:000786347600001
%N 8
%P 2560 [16 ]
%R 10.3390/molecules27082560
%U https://www.documentation.ird.fr/hor/fdi:010084689
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-06/010084689.pdf
%V 27
%W Horizon (IRD)
%X Herpes simplex type 2 (HSV-2) infection causes a significant life-long disease. Long-term side effects of antiviral drugs can lead to the emergence of drug resistance. Thus, propolis, a natural product derived from beehives, has been proposed to prevent or treat HSV-2 infections. Unfortunately, therapeutic applications of propolis are still limited due its poor solubility. To overcome this, a nanoparticle-based drug delivery system was employed. An ethanolic extract of propolis (EEP) was encapsulated in nanoparticles composed of poly(lactic-co-glycolic acid) and chitosan using a modified oil-in-water single emulsion by using the solvent evaporation method. The produced nanoparticles (EEP-NPs) had a spherical shape with a size of similar to 450 nm and presented satisfactory physicochemical properties, including positively charged surface (38.05 +/- 7.65 mV), high entrapment efficiency (79.89 +/- 13.92%), and sustained release profile. Moreover, EEP-NPs were less cytotoxic on Vero cells and exhibited anti-HSV-2 activity. EEP-NPs had a direct effect on the inactivation of viral particles, and also disrupted the virion entry and release from the host cells. A significant decrease in the expression levels of the HSV-2 replication-related genes (ICP4, ICP27, and gB) was also observed. Our study suggests that EEP-NPs provide a strong anti-HSV-2 activity and serve as a promising platform for the treatment of HSV-2 infections.
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