@article{fdi:010084689,
  title = {{A}ctivity of propolis nanoparticles against {HSV}-2 : promising approach to inhibiting infection and replication},
  author = {{S}angboonruang, {S}. and {S}emakul, {N}. and {S}ookkree, {S}. and {K}antapan, {J}. and {N}go-{G}iang-{H}uong, {N}icole and {K}hamduang, {W}. and {K}ongyai, {N}. and {T}ragoolpua, {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}erpes simplex type 2 ({HSV}-2) infection causes a significant life-long disease. {L}ong-term side effects of antiviral drugs can lead to the emergence of drug resistance. {T}hus, propolis, a natural product derived from beehives, has been proposed to prevent or treat {HSV}-2 infections. {U}nfortunately, therapeutic applications of propolis are still limited due its poor solubility. {T}o overcome this, a nanoparticle-based drug delivery system was employed. {A}n ethanolic extract of propolis ({EEP}) was encapsulated in nanoparticles composed of poly(lactic-co-glycolic acid) and chitosan using a modified oil-in-water single emulsion by using the solvent evaporation method. {T}he produced nanoparticles ({EEP}-{NP}s) had a spherical shape with a size of similar to 450 nm and presented satisfactory physicochemical properties, including positively charged surface (38.05 +/- 7.65 m{V}), high entrapment efficiency (79.89 +/- 13.92%), and sustained release profile. {M}oreover, {EEP}-{NP}s were less cytotoxic on {V}ero cells and exhibited anti-{HSV}-2 activity. {EEP}-{NP}s had a direct effect on the inactivation of viral particles, and also disrupted the virion entry and release from the host cells. {A} significant decrease in the expression levels of the {HSV}-2 replication-related genes ({ICP}4, {ICP}27, and g{B}) was also observed. {O}ur study suggests that {EEP}-{NP}s provide a strong anti-{HSV}-2 activity and serve as a promising platform for the treatment of {HSV}-2 infections.},
  keywords = {herpes simplex virus type 2 ; antiviral activity ; propolis ; chitosan ; poly(lactic-co-glycolic acid) ; polymeric nanoparticles},
  booktitle = {},
  journal = {{M}olecules},
  volume = {27},
  numero = {8},
  pages = {2560 [16 p.]},
  year = {2022},
  DOI = {10.3390/molecules27082560},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084689},
}