@article{fdi:010084616,
  title = {{U}sing a pupal exuvia to designate the undamaged neotype of a species belonging to a complex of sibling species : the case of {A}edes coluzzii ({D}iptera, {C}ulicidae)},
  author = {{B}erger, {A}udric and {L}e {G}off, {G}ilbert and {B}ouss{\`e}s, {P}hilippe and {R}ahola, {N}il and {F}erre, {J}. {B}. and {A}yala, {D}iego and {R}obert, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he mosquito species {A}edes ({O}chlerotatus) coluzzii {R}ioux, {G}uilvard & {P}asteur, 1998 was distinguished from its sibling species {A}edes detritus ({H}aliday, 1833) using an isoenzymatic method that required the destruction of the entire specimen, therefore no holotype was designated by the species authors. {W}e aimed to designate a neotype for {A}e. coluzzii from specimens collected from the type-locality and individually reared up to adult stage. {G}enomic {DNA} was extracted from pupal exuvia and {ITS}2 was sequenced, enabling verification of the identity of each specimen as {A}e. coluzzii or {A}e. detritus. {A}mong the series of {A}e. coluzzii, a male was designated as neotype and deposited in a collection. {T}o our knowledge, this is the first time the type of a mosquito species is deposited thanks to its molecular identification from its pupal exuvia. {T}he set of identified specimens allowed additional phylogenetic and morphologic studies.},
  keywords = {{M}osquito ; {N}eotype ; {M}olecular identification ; {S}pecies complex ; {A}edini ; {A}edes detritus ; {FRANCE} ; {CAMARGUE} ; {SALIN} {DE} {GIRAUD}},
  booktitle = {},
  journal = {{P}arasite},
  volume = {29},
  numero = {},
  pages = {19 [7 ]},
  ISSN = {1252-607{X}},
  year = {2022},
  DOI = {10.1051/parasite/2022020},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084616},
}