Van der Ent A. auteur aut IRD Pillon Yohan auteur aut IRD Fogliani B. auteur aut IRD Gei V. auteur aut IRD Jaffre T. auteur aut IRD Erskine P. D. auteur aut IRD Echevarria G. auteur aut IRD Spiers K. M. auteur aut IRD Paul A. L. D. auteur aut IRD Isnard Sandrine auteur aut IRD text journalArticle eng text/pdf reformatted digital access Purpose The Cunoniaceae are a major component of the New Caledonian flora with 91 endemic species that are highly unusual in that multiple metals are hyperaccumulated in different species. This makes it an ideal model system for studying the nature of the hyperaccumulation phenomenon. Methods X-ray fluorescence spectroscopy (XRF) scanning of all herbarium collections of the Cunoniaceae was undertaken at the Herbarium of New Caledonia to reveal incidences of nickel (Ni) and manganese (Mn) accumulation. Following on, the Mn hyperaccumulating P. reticulata and the Ni hyperaccumulating P. xaragurensis were selected for detailed follow-up investigations using synchrotron-based X-ray fluorescence microscopy (XFM). Results The systematic XRF screening of herbarium specimens showed that numerous species have high foliar Mn and Ni with species either accumulating Ni or Mn, but not both elements simultaneously. Soil 'extractable' Mn and Ni concentrations associated with Pancheria reticulata and P. xaragurensis greatly varies between the species. The XFM data shows that P. reticulata has a distinctive distribution pattern with Mn concentrated in large hypodermal cells. This contrasts with P. xaragurensis where Ni was mainly localized in and around the epidermis, and hypodermal cells were not observed. Conclusions Manganese and Ni accumulation are differently localized in Pancheria species growing on ultramafic soils, which is not explained by contrasting soils conditions, but represents different ecophysiological adaptations. specialized Cunoniaceae Elemental mapping Hyperaccumulator Manganese Nickel New Caledonia Ultramafic soils XRF NOUVELLE CALEDONIE 076 068 [Early access] [20 ] 2022 0032-079X https://www.documentation.ird.fr/hor/fdi:010084613 10.1007/s11104-022-05388-3 0032-079X [F B010084613] https://www.documentation.ird.fr/hor/fdi:010084613 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2022-05/010084613.pdf IRD - Base Horizon / Pleins textes 2022-05-04 2025-02-24 fdi:010084613 fre