Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti Zouache K. auteur aut IRD Martin E. auteur aut IRD Rahola Nil auteur aut IRD Gangue M. F. auteur aut IRD Minard G. auteur aut IRD Dubost A. auteur aut IRD Tran Van V. auteur aut IRD Dickson L. auteur aut IRD Ayala Diego auteur aut IRD Lambrechts L. auteur aut IRD Valiente Moro C. auteur aut IRD text journalArticle eng text/pdf born digital access Higher similarity in bacterial and fungal community structure in larvae and the water of their breeding sites than between larvae from different larval habitats. Mosquito larvae are naturally exposed to microbial communities present in a variety of larval development sites. Several earlier studies have highlighted that the larval habitat influences the composition of the larval bacterial microbiota. However, little information is available on their fungal microbiota, i.e. the mycobiota. In this study, we provide the first simultaneous characterization of the bacterial and fungal microbiota in field-collected Aedes aegypti larvae and their respective aquatic habitats. We evaluated whether the microbial communities associated with the breeding site may affect the composition of both the bacterial and fungal communities in Ae. aegypti larvae. Our results show a higher similarity in microbial community structure for both bacteria and fungi between larvae and the water in which larvae develop than between larvae from different breeding sites. This supports the hypothesis that larval habitat is a major factor driving microbial composition in mosquito larvae. Since the microbiota plays an important role in mosquito biology, unravelling the network of interactions that operate between bacteria and fungi is essential to better understand the functioning of the mosquito holobiont. specialized mosquito microbiota larva breeding site bacteria mycobiota 052 084 FEMS Microbiology Ecology 98 1 fiac016 [11 ] 2022 0168-6496 https://www.documentation.ird.fr/hor/fdi:010084524 10.1093/femsec/fiac016 0168-6496 [F B010084524] https://www.documentation.ird.fr/hor/fdi:010084524 https://www.documentation.ird.fr/intranet/publi/2022-04/010084524.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2022-04-06 2025-02-24 fdi:010084524 fre