@article{fdi:010084499,
  title = {{N}ettle manure : an unsuspected source of bacteriophages active against various phytopathogenic bacteria},
  author = {{B}oyer, {M}. and {W}isniewski-{D}ye, {F}. and {C}ombrisson, {J}. and {B}ally, {R}. and {D}uponnois, {R}obin and {C}ostechareyre, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}creening of 10 environmental samples (mainly of rhizospheric origin) for lytic activity against two bacterial phytopathogens, {P}seudomonas syringae pv. tomato {DC}3000 ({CFBP}2212) and {X}anthomonas hortorum pv. vitians ({CFBP}3979), revealed that four samples harboured phages that were active against one strain. {O}nly one sample, composed of an artisanal nettle liquid manure, contained phages able to lyse both strains. {E}lectron microscopy revealed the presence of tailed bacteriophages, with all phages isolated on the {X}anthomonas strain displaying a contractile tail typical of members of the family {M}yoviridae, whereas phages isolated on the {P}seudomonas strain were related to members of the family {S}iphoviridae and short-tailed members of the family {P}odoviridae. {S}equence analysis of the two {P}odoviridae-like bacteriophages isolated on {P}seudomonas syringae pv. tomato, {P}st_{GM}1 isolated from nettle manure and {P}st_{GIL}1 isolated from infected lettuce leaves, revealed (i) strong homology between the two isolated phages, (ii) a high degree of sequence similarity to various phages isolated from various environments and from different geographical locations, and (iii) similarity of these phages to members of the family {A}utographiviridae, and more precisely, the genus {G}hunavirus. {F}urther investigation of the potential of nettle manure to host phages that could be active against a wider range of strains revealed that it contained phages active against 10 phytopathogens (out of 16 tested). {T}hus, nettle manure (and likely other plant manures) could represent a valuable source of phages, especially those targeting bacterial phytopathogens, in the same way that anthropized environments such as sewage are widely used as sources of phages active against opportunistic or acute pathogens of humans.},
  keywords = {},
  booktitle = {},
  journal = {{A}rchives of {V}irology},
  volume = {[{E}arly access]},
  numero = {},
  pages = {[12 p.]},
  ISSN = {0304-8608},
  year = {2022},
  DOI = {10.1007/s00705-022-05391-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084499},
}