@article{fdi:010084492,
  title = {{E}ffects of an alphasatellite on the life cycle of the nanovirus {F}aba bean necrotic yellows virus},
  author = {{M}ansourpour, {M}. and {G}allet, {R}. and {A}bbasi, {A}. and {B}lanc, {S}. and {D}izadji, {A}. and {Z}eddam, {J}ean-{L}ouis},
  editor = {},
  language = {{ENG}},
  abstract = {{N}anoviruses are plant viruses with a multipartite single-stranded {DNA} (ss{DNA}) genome. {A}lphasatellites are commonly associated with nanovirus infections, but their putative impact on their helper viruses is unknown. {I}n this study, we investigated the role of subterranean clover stunt alphasatellite 1 (here named {SCSA} 1) on various important traits of {F}aba bean necrotic yellows virus ({FBNYV}) in its host plant {V}icia faba and aphid vector {A}cyrthosiphon pisum, including disease symptoms, viral accumulation, and viral transmission. {T}he results indicate that {SCSA} 1 does not affect the severity of symptoms nor overall {FBNYV} accumulation in {V}. faba, but it does change the relative amounts of its different genomic segments. {M}oreover, the association of {SCSA} 1 with {FBNYV} increases the rate of plant-to-plant transmission by a process seemingly unrelated to the simple increase of viral accumulation in the vector. {T}hese results represent the first study on the impact of an alphasatellite on the biology of its helper nanovirus. {T}hey suggest that {SCSA} 1 may benefit {FBNYV}, but the genericity of this conclusion is discussed and questioned. {IMPORTANCE} {A}lphasatellites are circular single-stranded {DNA} molecules frequently found in association with natural isolates of nanoviruses and some geminiviruses, the two ss{DNA} plant-infecting virus families. {W}hile the implications of alphasatellite presence in geminivirus infections are relatively well documented, comparable studies on alphasatellites associated with nanoviruses are not available. {H}ere, we confirm that subterranean clover stunt alphasatellite 1 affects different traits of its helper nanovirus, {F}aba bean necrotic yellows virus, both in the host plant and aphid vector. {W}e show that the frequencies of the virus segments change in the presence of alphasatellite, in both the plant and the vector. {W}e also confirm that although within-plant virus load and symptoms are not affected by alphasatellite, the presence of alphasatellite decreases within-aphid virus load but significantly increases virus transmission rate, and thus it may confer a possible evolutionary advantage for the helper virus.},
  keywords = {{N}anovirus ; alphasatellite ; {SCSA} 1 ; genome formula ; virus accumulation ; aphid transmission ; {A}cyrthosiphon pisum ; multipartite virus},
  booktitle = {},
  journal = {{J}ournal of {V}irology},
  volume = {96},
  numero = {3},
  pages = {e01388--21 [12 p.]},
  ISSN = {0022-538{X}},
  year = {2022},
  DOI = {10.1128/{JVI}.01388-21},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084492},
}