@article{fdi:010084357,
  title = {{T}ranscriptome analysis of an {A}edes albopictus cell line single- and dual-infected with {L}ammi virus and {WNV}},
  author = {{O}hlund, {P}. and {D}elhomme, {N}. and {H}ayer, {J}uliette and {H}esson, {J}. {C}. and {B}lomstrom, {A}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{U}nderstanding the flavivirus infection process in mosquito hosts is important and fundamental in the search for novel control strategies that target the mosquitoes' ability to carry and transmit pathogenic arboviruses. {A} group of viruses known as insect-specific viruses ({ISV}s) has been shown to interfere with the infection and replication of a secondary arbovirus infection in mosquitoes and mosquito-derived cell lines. {H}owever, the molecular mechanisms behind this interference are unknown. {T}herefore, in the present study, we infected the {A}edes albopictus cell line {U}4.4 with either the {W}est {N}ile virus ({WNV}), the insect-specific {L}ammi virus ({L}am{V}) or an infection scheme whereby cells were pre-infected with {L}am{V} 24 h prior to {WNV} challenge. {T}he q{PCR} analysis showed that the dual-infected {U}4.4 cells had a reduced number of {WNV} {RNA} copies compared to {WNV}-only infected cells. {T}he transcriptome profiles of the different infection groups showed a variety of genes with altered expression. {WNV}-infected cells had an up-regulation of a broad range of immune-related genes, while in {L}am{V}-infected cells, many genes related to stress, such as different heat-shock proteins, were up-regulated. {T}he transcriptome profile of the dual-infected cells was a mix of up- and down-regulated genes triggered by both viruses. {F}urthermore, we observed an up-regulation of signal peptidase complex ({SPC}) proteins in all infection groups. {T}hese {SPC} proteins have shown importance for flavivirus assembly and secretion and could be potential targets for gene modification in strategies for the interruption of flavivirus transmission by mosquitoes.},
  keywords = {insect-specific flaviviruses ; {W}est {N}ile virus ; {A}edes albopictus ; transcriptome ; viral interference},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {M}olecular {S}ciences},
  volume = {23},
  numero = {2},
  pages = {875 [17 p.]},
  year = {2022},
  DOI = {10.3390/ijms23020875},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084357},
}