@article{fdi:010084309,
  title = {{D}engue virus infection modifies mosquito blood-feeding behavior to increase transmission to the host},
  author = {{X}iang, {B}. {W}. {W}. and {S}aron, {W}. {A}. {A}. and {S}tewart, {J}. {C}. and {H}ain, {A}. and {W}alvekar, {V}. and {M}iss{\'e}, {D}oroth{\'e}e and {T}homas, {F}. and {K}ini, {R}. {M}. and {R}oche, {B}enjamin and {C}laridge-{C}hang, {A}. and {S}t {J}ohn, {A}. {L}. and {P}ompon, {J}ulien},
  editor = {},
  language = {{ENG}},
  abstract = {{M}osquito blood-feeding behavior is a key determinant of the epidemiology of dengue viruses ({DENV}), the most-prevalent mosquitoborne viruses. {H}owever, despite its importance, how {DENV} infection influences mosquito blood-feeding and, consequently, transmission remains unclear. {H}ere, we developed a high-resolution, video-based assay to observe the blood-feeding behavior of {A}edes aegypti mosquitoes on mice. {W}e then applied multivariate analysis on the high-throughput, unbiased data generated from the assay to ordinate behavioral parameters into complex behaviors. {W}e showed that {DENV} infection increases mosquito attraction to the host and hinders its biting efficiency, the latter resulting in the infected mosquitoes biting more to reach similar blood repletion as uninfected mosquitoes. {T}o examine how increased biting influences {DENV} transmission to the host, we established an in vivo transmission model with immuno-competent mice and demonstrated that successive short probes result in multiple transmissions. {F}inally, to determine how {DENV}-induced alterations of hostseeking and biting behaviors influence dengue epidemiology, we integrated the behavioral data within a mathematical model. {W}e calculated that the number of infected hosts per infected mosquito, as determined by the reproduction rate, tripled when mosquito behavior was influenced by {DENV} infection. {T}aken together, this multidisciplinary study details how {DENV} infection modulates mosquito blood-feeding behavior to increase vector capacity, proportionally aggravating {DENV} epidemiology. {B}y elucidating the contribution of mosquito behavioral alterations on {DENV} transmission to the host, these results will inform epidemiological modeling to tailor improved interventions against dengue.},
  keywords = {mosquito ; dengue virus ; blood-feeding behavior ; transmission ; epidemiology},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {119},
  numero = {3},
  pages = {e2117589119 [9 ]},
  ISSN = {0027-8424},
  year = {2022},
  DOI = {10.1073/pnas.2117589119},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084309},
}