@article{fdi:010084282,
  title = {{P}roteomic analysis of the promastigote secretome of seven {L}eishmania species},
  author = {{P}issarra, {J}oana and {P}agniez, {J}ulie and {P}etitdidier, {E}lodie and {S}eveno, {M}. and {V}igy, {O}. and {B}ras {G}oncalves, {R}achel and {L}emesre, {J}ean-{L}oup and {H}olzmuller, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}eishmaniasis is one of the most impactful parasitic diseases worldwide, endangering the lives of 1 billion people every year. {T}here are 20 different species of {L}eishmania able to infect humans, causing cutaneous ({CL}), visceral ({VL}), and/or mucocutaneous leishmaniasis ({MCL}). {L}eishmania parasites are known to secrete a plethora of proteins to establish infection and modulate the host's immune system. {I}n this study, we analyzed using tandem mass spectrometry the total protein content of the secretomes produced by promastigote forms from seven {L}eishmania species grown in serum-free in vitro cultures. {T}he core secretome shared by all seven {L}eishmania species corresponds to up to one-third of total secreted proteins, suggesting conserved mechanisms of adaptation to the vertebrate host. {T}he relative abundance confirms the importance of known virulence factors and some proteins uniquely present in {CL}-or {VL}-causing species and may provide further insight regarding their pathogenesis. {B}ioinformatic analysis showed that most proteins were secreted via unconventional mechanisms, with an important role for vesicle based secretion for all species. {G}ene {O}ntology annotation and enrichment analyses showed a high level of functional conservation among species. {T}his study contributes to the current knowledge on the biological significance of differently secreted proteins and provides new information on the correlation of {L}eishmania secretome to clinical outcomes and species-specific pathogenesis.},
  keywords = {leishmaniasis ; {L}eishmania ; promastigote ; secretome ; mass spectrometry},
  booktitle = {},
  journal = {{J}ournal of {P}roteome {R}esearch},
  volume = {21},
  numero = {1},
  pages = {30--48},
  ISSN = {1535-3893},
  year = {2022},
  DOI = {10.1021/acs.jproteome.1c00244},
  URL = {https://www.documentation.ird.fr/hor/fdi:010084282},
}